ArticleProceedings of the National Academy of Sciences of the United States of America2000
Ca2+ channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and abscisic acid.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed, 350 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- Synthetic ion channel inhibitors enhance plant drought tolerance.Nature communications · 2026Article
- Linking redox and calcium signaling via thioredoxins.Journal of experimental botany · 2026Review
- A drought stress-responsive metabolite malate modulates stomatal responses through G-protein-dependent pathway in grapevine and Arabidopsis.Molecular horticulture · 2026Article
- Dual function of overexpressing plasma membrane HScience advances · 2024Article
- Article
- OPEN STOMATA 1 phosphorylates CYCLIC NUCLEOTIDE-GATED CHANNELs to trigger Ca2+ signaling for abscisic acid-induced stomatal closure in Arabidopsis.The Plant cell · 2024Article
- Function and Mechanism of Abscisic Acid on Microglia-Induced Neuroinflammation in Parkinson's Disease.International journal of molecular sciences · 2024Review
- A charged existence: A century of transmembrane ion transport in plants.Plant physiology · 2024Review
- OnGuard3e: A predictive, ecophysiology-ready tool for gas exchange and photosynthesis research.Plant, cell & environment · 2023Article
- Article
- Stomatal Responses of Two Drought-Tolerant Barley Varieties with Different ROS Regulation Strategies under Drought Conditions.Antioxidants (Basel, Switzerland) · 2023Article
- Effect of Abscisic Acid on Growth, Fatty Acid Profile, and Pigment Composition of the ChlorophyteLife (Basel, Switzerland) · 2023Article
- Plant hormone regulation of abiotic stress responses.Nature reviews. Molecular cell biology · 2022Review
- Green Tea Catechins, (-)-Catechin Gallate, and (-)-Gallocatechin Gallate are Potent Inhibitors of ABA-Induced Stomatal Closure.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Distinct Roles for KASH Proteins SINE1 and SINE2 in Guard Cell Actin Reorganization, Calcium Oscillations, and Vacuolar Remodeling.Frontiers in plant science · 2022Article
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- The signatures of organellar calcium.Plant physiology · 2021Review
- The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress.BMC genomics · 2021Article
- Membrane voltage as a dynamic platform for spatiotemporal signaling, physiological, and developmental regulation.Plant physiology · 2021Article
64 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In stomatal guard cells of higher-plant leaves, abscisic acid (ABA) evokes increases in cytosolic free Ca(2+) concentration ([Ca(2+)](i)) by means of Ca(2+) entry from outside and release from intracellular stores. The mechanism(s) for Ca(2+) flux across the plasma membrane is poorly understood. Because [Ca(2+)](i) increases are voltage-sensitive, we suspected a Ca(2+) channel at the guard cell plasma membrane that activates on hyperpolarization and is regulated by ABA. We recorded single-channel currents across the Vicia guard cell plasma membrane using Ba(2+) as a charge-carrying ion. Both cell-attached and excised-patch measurements uncovered single-channel events with a maximum conductance of 12.8 +/- 0.4 pS and a high selectivity for Ba(2+) (and Ca(2+)) over K(+) and Cl(-). Unlike other Ca(2+) channels characterized to date, these channels rectified strongly toward negative voltages with an open probability (P(o)) that increased with [Ba(2+)] outside and decreased roughly 10-fold when [Ca(2+)](i) was raised from 200 nM to 2 microM. Adding 20 microM ABA increased P(o), initially by 63- to 260-fold; in both cell-attached and excised patches, it shifted the voltage sensitivity for channel activation, and evoked damped oscillations in P(o) with periods near 50 s. A similar, but delayed response was observed in 0.1 microM ABA. These results identify a Ca(2+)-selective channel that can account for Ca(2+) influx and increases in [Ca(2+)](i) triggered by voltage and ABA, and they imply a close physical coupling at the plasma membrane between ABA perception and Ca(2+) channel control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.