ArticleProceedings of the National Academy of Sciences of the United States of America2000
Development of an extrachromosomal cloning vector system for use in Borrelia burgdorferi.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 72 citations in OpenAlex.
- Hitchhiker's Guide toJournal of bacteriology · 2024Review
- Development and validation of systems for genetic manipulation of the Old World tick-borne relapsing fever spirochete, Borrelia duttonii.PLoS neglected tropical diseases · 2024Article
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi.Applied and environmental microbiology · 2018Article
- Genetic Transformation and Complementation.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Identification of three extra-chromosomal replicons in Leptospira pathogenic strain and development of new shuttle vectors.BMC genomics · 2015Article
- Use of an endogenous plasmid locus for stable in trans complementation in Borrelia burgdorferi.Applied and environmental microbiology · 2015Article
- Genetics of Borrelia burgdorferi.Annual review of genetics · 2012Review
- The coenzyme A disulphide reductase of Borrelia burgdorferi is important for rapid growth throughout the enzootic cycle and essential for infection of the mammalian host.Molecular microbiology · 2011Article
- CheY3 of Borrelia burgdorferi is the key response regulator essential for chemotaxis and forms a long-lived phosphorylated intermediate.Journal of bacteriology · 2011Article
- Genetic transformation of Borrelia burgdorferi.Current protocols in microbiology · 2011Article
- lacZ reporter system for use in Borrelia burgdorferi.Applied and environmental microbiology · 2010Article
- Use of rpsL as a Counterselectable Marker in Borrelia burgdorferi.Applied and environmental microbiology · 2010Article
- The flat-ribbon configuration of the periplasmic flagella of Borrelia burgdorferi and its relationship to motility and morphology.Journal of bacteriology · 2009Article
- Essential role of the response regulator Rrp2 in the infectious cycle of Borrelia burgdorferi.Infection and immunity · 2008Article
- Real-time high resolution 3D imaging of the lyme disease spirochete adhering to and escaping from the vasculature of a living host.PLoS pathogens · 2008Article
- Regulation of expression of the fibronectin-binding protein BBK32 in Borrelia burgdorferi.Journal of bacteriology · 2007Article
- Identification of specific chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay.Applied and environmental microbiology · 2007Article
- Biological basis for syphilis.Clinical microbiology reviews · 2006Review
- Alternate sigma factor RpoS is required for the in vivo-specific repression of Borrelia burgdorferi plasmid lp54-borne ospA and lp6.6 genes.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Molecular genetic analysis of Borrelia burgdorferi, the cause of Lyme disease, has been hampered by the absence of any means of efficient generation, identification, and complementation of chromosomal and plasmid null gene mutants. The similarity of borrelial G + C content to that of Gram-positive organisms suggested that a wide-host-range plasmid active in Gram-positive bacteria might also be recognized by borrelial DNA replication machinery. One such plasmid, pGK12, is able to propagate in both Gram-positive and Gram-negative bacteria and carries erythromycin and chloramphenicol resistance markers. pGK12 propagated extrachromosomally in B. burgdorferi B31 after electroporation but conferred only erythromycin resistance. pGK12 was used to express enhanced green fluorescent protein in B31 under the control of the flaB promoter. Escherichia coli transformed with pGK12 DNA extracted from B31 expressing only erythromycin resistance developed both erythromycin and chloramphenicol resistance, and plasmid DNA isolated from these transformed E. coli had a restriction pattern similar to the original pGK12. Our data indicate that the replicons of pGK12 can provide the basis to continue developing efficient genetic systems for B. burgdorferi together with the erythromycin resistance and reporter egfp genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.