ArticleProceedings of the National Academy of Sciences of the United States of America2000
Prolyl 4-hydroxylase is required for viability and morphogenesis in Caenorhabditis elegans.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed.
- Threonine Phosphorylation Is a Bioreversible Surrogate of Proline Hydroxylation in a Collagen Triple Helix.Journal of the American Chemical Society · 2026Article
- P4HA2 Participates in Pathogenesis of Refractive Error by Regulating Collagen Posttranslational Modification and Extracellular Matrix Balance.Human mutation · 2026Article
- A collagen amino acid composition supplementation reduces biological age in humans and increases health and lifespan in vivo.npj aging · 2025Article
- Calcium-binding protein CALU-1 is essential for proper collagen formation in Caenorhabditis elegans.Cellular and molecular life sciences : CMLS · 2025Article
- The Caenorhabditis elegans cuticle and precuticle: a model for studying dynamic apical extracellular matrices in vivo.Genetics · 2024Review
- Systematic analysis and expression of Gossypium 2ODD superfamily highlight the roles of GhLDOXs responding to alkali and other abiotic stress in cotton.BMC plant biology · 2023Article
- HypDB: A functionally annotated web-based database of the proline hydroxylation proteome.PLoS biology · 2022Article
- Article
- Prolyl 4-Hydroxylase: Substrate Isosteres in Which an (E)- or (Z)-Alkene Replaces the Prolyl Peptide Bond.Biochemistry · 2017Article
- 4-Fluoroprolines: Conformational Analysis and Effects on the Stability and Folding of Peptides and Proteins.Topics in heterocyclic chemistry · 2017Article
- Article
- The invertebrate Caenorhabditis elegans biosynthesizes ascorbate.Archives of biochemistry and biophysics · 2015Article
- Regulation of axonal midline guidance by prolyl 4-hydroxylation in Caenorhabditis elegans.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Bioavailable affinity label for collagen prolyl 4-hydroxylase.Bioorganic & medicinal chemistry · 2013Article
- Prolyl 4 hydroxylase: a critical target in the pathophysiology of diseases.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2013Article
- COL4A1 and COL4A2 mutations and disease: insights into pathogenic mechanisms and potential therapeutic targets.Human molecular genetics · 2012Review
- Genetic interaction between Caenorhabditis elegans teneurin ten-1 and prolyl 4-hydroxylase phy-1 and their function in collagen IV-mediated basement membrane integrity during late elongation of the embryo.Molecular biology of the cell · 2011Article
- Prolyl 4-hydroxylase.Critical reviews in biochemistry and molecular biology · 2010Review
- Article
- Origin of the stability conferred upon collagen by fluorination.Bioorganic & medicinal chemistry letters · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The genome of Caenorhabditis elegans possesses two genes, dpy-18 and phy-2, that encode alpha subunits of the enzyme prolyl 4-hydroxylase. We have generated deletions within each gene to eliminate prolyl 4-hydroxylase activity from the animal. The dpy-18 mutant has an aberrant body morphology, consistent with a role of prolyl 4-hydroxylase in formation of the body cuticle. The phy-2 mutant is phenotypically wild type. However, the dpy-18; phy-2 double mutant is not viable, suggesting an essential role for prolyl 4-hydroxylase that is normally accomplished by either dpy-18 or phy-2. The effects of the double mutation were mimicked by small-molecule inhibitors of prolyl 4-hydroxylase, validating the genetic results and suggesting that C. elegans can serve as a model system for the discovery of new inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.