ArticleProceedings of the National Academy of Sciences of the United States of America2000
A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 85 citations in OpenAlex.
- Identification of a novel bacteriophage attachment site into ffs, the 4.5S non-coding RNA component of the signal recognition particle.Scientific reports · 2025Article
- Development of luciferase-based highly sensitive reporters that detect ER-associated protein biogenesis abnormalities.iScience · 2024Article
- Development of a sensor for disulfide bond formation in diverse bacteria.Journal of bacteriology · 2024Article
- Genetic Analysis of Protein Translocation.The protein journal · 2019Review
- Inhibition of Pseudomonas aeruginosa and Mycobacterium tuberculosis disulfide bond forming enzymes.Molecular microbiology · 2019Article
- Compounds targeting disulfide bond forming enzyme DsbB of Gram-negative bacteria.Nature chemical biology · 2015Article
- Signal recognition particle and SecA cooperate during export of secretory proteins with highly hydrophobic signal sequences.PloS one · 2014Article
- Specificity of SecYEG for PhoA precursors and SecA homologs on SecA protein-conducting channels.Biochemical and biophysical research communications · 2013Article
- Structural basis of signal sequence surveillance and selection by the SRP-FtsY complex.Nature structural & molecular biology · 2013Article
- The variable subdomain of Escherichia coli SecA functions to regulate SecA ATPase activity and ADP release.Journal of bacteriology · 2012Article
- Translation elongation regulates substrate selection by the signal recognition particle.The Journal of biological chemistry · 2012Article
- Binding Protein-Dependent Uptake of Maltose into Cells via an ATP-Binding Cassette Transporter.EcoSal Plus · 2010Article
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- Inhibition of bacterial disulfide bond formation by the anticoagulant warfarin.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Genetic suppressors and recovery of repressed biochemical memory.The Journal of biological chemistry · 2009Review
- Transient ribosomal attenuation coordinates protein synthesis and co-translational folding.Nature structural & molecular biology · 2009Article
- Characterization of conserved bases in 4.5S RNA of Escherichia coli by construction of new F' factors.Journal of bacteriology · 2008Article
- Yersinia enterocolitica type III secretion of YopR requires a structure in its mRNA.Molecular microbiology · 2008Article
- A channel connecting the mother cell and forespore during bacterial endospore formation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We describe an Escherichia coli genetic screen that yields mutations affecting two different cellular processes: disulfide bond formation and membrane protein assembly. The mutants defective in disulfide bond formation include additional classes of dsbA and dsbB mutations. The membrane protein assembly defective mutants contain a mutation in the secA operon and three mutations in the ffs gene, which encodes 4.5S RNA. These latter mutations are the only ones to be isolated in a gene encoding a component of the bacterial signal recognition particle by screening in vivo for defects in membrane protein insertion. A sensitive method for examining membrane protein localization shows that the ffs and secA locus mutations affect membrane assembly of the polytopic membrane protein, MalF. The ffs mutations also affect the membrane insertion of the FtsQ and the AcrB proteins. Although both the ffs and the secA locus mutations interfere with membrane protein assembly, only the latter also reduces export of a protein containing a cleavable signal sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.