ArticleProceedings of the National Academy of Sciences of the United States of America2000
Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: application of a method called SERF.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Single-molecule force measurements show that r-proteins assist 23S rRNA co-transcriptional folding.Biophysical journal · 2026Article
- Mechanisms of Linezolid Resistance in Mycobacteria.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Non-Coding RNA-Driven Regulation of rRNA Biogenesis.International journal of molecular sciences · 2020Review
- Assessment of the nucleotide modifications in the high-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit.Nucleic acids research · 2020Article
- Force measurements show that uL4 and uL24 mechanically stabilize a fragment of 23S rRNA essential for ribosome assembly.RNA (New York, N.Y.) · 2019Article
- Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly.PLoS genetics · 2014Article
- Ribosomal RNAs are tolerant toward genetic insertions: evolutionary origin of the expansion segments.Nucleic acids research · 2008Article
- Selection for intragenic suppressors of lethal 23S rRNA mutations in Escherichia coli identifies residues important for ribosome assembly and function.Molecular genetics and genomics : MGG · 2007Article
- The N-terminal extension of Escherichia coli ribosomal protein L20 is important for ribosome assembly, but dispensable for translational feedback control.RNA (New York, N.Y.) · 2005Article
- Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX.Nucleic acids research · 2004Article
- The extended loops of ribosomal proteins L4 and L22 are not required for ribosome assembly or L4-mediated autogenous control.RNA (New York, N.Y.) · 2003Article
- RNA-structural mimicry in Escherichia coli ribosomal protein L4-dependent regulation of the S10 operon.The Journal of biological chemistry · 2003Article
- Ribosomal protein S15 represses its own translation via adaptation of an rRNA-like fold within its mRNA.The EMBO journal · 2003Article
- Point mutation in essential genes with loss or mutation of the second allele: relevance to the retention of tumor-specific antigens.The Journal of experimental medicine · 2001Article
- A short fragment of 23S rRNA containing the binding sites for two ribosomal proteins, L24 and L4, is a key element for rRNA folding during early assembly.RNA (New York, N.Y.) · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-thirds of the 54 proteins of the Escherichia coli ribosome interact directly with the rRNAs, but the rRNA binding sites of only a very few proteins are known. We present a method (selection of random RNA fragments; SERF) that can identify the minimal binding region for proteins within ribonucleo-protein complexes such as the ribosome. The power of the method is exemplified with the ribosomal proteins L4 and L6. Binding sequences are identified for both proteins and characterized by phosphorothioate footprinting. Surprisingly, the binding region of L4, a 53-nt rRNA fragment of domain I of 23S rRNA, can simultaneously and independently bind L24, one of the two assembly initiator proteins of the large subunit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.