ArticleProceedings of the National Academy of Sciences of the United States of America2000
5-methylcytosine-DNA glycosylase activity is present in a cloned G/T mismatch DNA glycosylase associated with the chicken embryo DNA demethylation complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 144 citations in OpenAlex.
- Targeted DNA Demethylation: Vectors, Effectors and Perspectives.Biomedicines · 2023Review
- DNA methylation dynamics of genomic imprinting in mouse development.Biology of reproduction · 2018Review
- Methylation-Demethylation Dynamics: Implications of Changes in Acute Kidney Injury.Analytical cellular pathology (Amsterdam) · 2018Review
- Multiple biological functions of transcription factor 21 in the development of various cancers.OncoTargets and therapy · 2018Review
- The Role of Activity-Dependent DNA Demethylation in the Adult Brain and in Neurological Disorders.Frontiers in molecular neuroscience · 2018Review
- Role of Base Excision "Repair" Enzymes in Erasing Epigenetic Marks from DNA.Chemical reviews · 2016Review
- Neil DNA glycosylases promote substrate turnover by Tdg during DNA demethylation.Nature structural & molecular biology · 2016Article
- Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.Chemical research in toxicology · 2015Article
- The epigenetics of aging and neurodegeneration.Progress in neurobiology · 2015Review
- Role of base excision repair in maintaining the genetic and epigenetic integrity of CpG sites.DNA repair · 2015Review
- Uracil-DNA glycosylase is involved in DNA demethylation and required for embryonic development in the zebrafish embryo.The Journal of biological chemistry · 2014Article
- Induced DNA demethylation by targeting Ten-Eleven Translocation 2 to the human ICAM-1 promoter.Nucleic acids research · 2014Article
- Article
- Active DNA demethylation in post-mitotic neurons: a reason for optimism.Neuropharmacology · 2013Review
- Catalog of mRNA expression patterns for DNA methylating and demethylating genes in developing mouse lower urinary tract.Gene expression patterns : GEP · 2013Article
- Specific transcriptional enhancement of inducible nitric oxide synthase by targeted promoter demethylation.Epigenetics · 2013Article
- Mammalian DNA demethylation: multiple faces and upstream regulation.Epigenetics · 2013Article
- DNA 5-methylcytosine demethylation activities of the mammalian DNA methyltransferases.The Journal of biological chemistry · 2013Article
- DNA demethylation pathways: recent insights.Genetics & epigenetics · 2013Review
- DNA demethylation by TDG.Epigenomics · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously have shown that DNA demethylation by chicken embryo 5-methylcytosine DNA glycosylase (5-MCDG) needs both RNA and proteins. One of these proteins is a RNA helicase. Further peptides were sequenced, and three of them are identical to the mammalian G/T mismatch DNA glycosylase. A 3,233-bp cDNA coding for the chicken homologue of human G/T mismatch DNA glycosylase was isolated and sequenced. The derived amino acid sequence (408 aa) shows 80% identity with the human G/T mismatch DNA glycosylase, and both the C and N-terminal parts have about 50% identity. As for the highly purified chicken embryo DNA demethylation complex the recombinant protein expressed in Escherichia coli has both G/T mismatch and 5-MCDG activities. The recombinant protein has the same substrate specificity as the chicken embryo 5-MCDG where hemimethylated DNA is a better substrate than symmetrically methylated CpGs. The activity ratio of G/T mismatch and 5-MCDG is about 30:1 for the recombinant protein expressed in E. coli and 3:1 for the purified enzyme from chicken embryos. The incubation of a recombinant CpG-rich RNA isolated from the purified DNA demethylation complex with the recombinant enzyme strongly inhibits G/T mismatch glycosylase while slightly stimulating the activity of 5-MCDG. Deletion mutations indicate that G/T mismatch and 5-MCDG activities share the same areas of the N- and C-terminal parts of the protein. In reconstitution experiments RNA helicase in the presence of recombinant RNA and ATP potentiates the activity of 5-MCDG.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.