ArticleMolecular and cellular biology2000
Distinct phosphoisoforms of the Xenopus Mcm4 protein regulate the function of the Mcm complex.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Signs of carcinogenicity induced by parathion, malathion, and estrogen in human breast epithelial cells (Review).Oncology reports · 2021Article
- Cdc7 kinase stimulates Aurora B kinase in M-phase.Scientific reports · 2019Article
- Phosphorylation of Minichromosome Maintenance 3 (MCM3) by Checkpoint Kinase 1 (Chk1) Negatively Regulates DNA Replication and Checkpoint Activation.The Journal of biological chemistry · 2015Article
- Xenopus Cdc7 executes its essential function early in S phase and is counteracted by checkpoint-regulated protein phosphatase 1.Open biology · 2014Article
- Genomic instability in cancer.Cold Spring Harbor perspectives in biology · 2013Review
- Genome wide comparative comprehensive analysis of Plasmodium falciparum MCM family with human host.Communicative & integrative biology · 2012Article
- MCM2-7 form double hexamers at licensed origins in Xenopus egg extract.The Journal of biological chemistry · 2011Article
- Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload.The Journal of cell biology · 2011Article
- Cyclin A promotes S-phase entry via interaction with the replication licensing factor Mcm7.Molecular and cellular biology · 2011Article
- Characterization of Leishmania donovani MCM4: expression patterns and interaction with PCNA.PloS one · 2011Article
- Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex.Nucleic acids research · 2010Article
- A genetic screen for replication initiation defective (rid) mutants in Schizosaccharomyces pombe.Cell division · 2010Article
- How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?Genes & development · 2010Review
- Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation.Journal of biomedical science · 2010Article
- Geminin stabilizes Cdt1 during meiosis in Xenopus oocytes.The Journal of biological chemistry · 2009Article
- Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.Molecular biology of the cell · 2008Article
- Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication.Genes & development · 2005Article
- Cyclin/CDK regulates the nucleocytoplasmic localization of the human papillomavirus E1 DNA helicase.Journal of virology · 2004Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Human cytomegalovirus infection leads to accumulation of geminin and inhibition of the licensing of cellular DNA replication.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initiation of DNA replication in eukaryotes requires the assembly of prereplication complexes (pre-Rcs) at the origins of replication. The assembly and function of the pre-Rcs appear to be controlled by phosphorylation events. In this study we report the detailed characterization of the cell cycle phosphorylation of one component of the Xenopus pre-Rcs, the Mcm protein complex. We show that individual Mcm subunits are differentially phosphorylated during the cell cycle. During mitosis, the Mcm4 subunit is hyperphosphorylated, while the other subunits are not actively phosphorylated. The mitotic phosphorylation of Mcm4 requires Cdc2-cyclin B and other unknown kinases. Following exit from mitosis, the Mcm4 subunit of the cytosolic interphase complex undergoes dephosphorylation, and the Mcm2, Mcm3, or Mcm6 subunits are then actively phosphorylated by kinase(s) other than cyclin-dependent kinases (Cdks) or Cdc7. The association of the Mcm complex with the pre-Rcs correlates with the formation of a transient interphase complex. This complex contains an intermediately phosphorylated Mcm4 subunit and is produced by partial dephosphorylation of the mitotic hyperphosphorylated Mcm4 protein. Complete dephosphorylation of the Mcm4 subunit inactivates the Mcm complex and prevents its binding to the chromatin. Once the Mcm complex is assembled on the chromatin the Mcm4 and the Mcm2 proteins are the only subunits phosphorylated during the activation of the pre-Rcs. These chromatin-associated phosphorylations require nuclear transport and are independent of Cdk2-cyclin E. These results suggest that the changes in Mcm4 phosphorylation regulate pre-Rc assembly and the function of the pre-Rcs on the chromatin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.