ArticleMolecular and cellular biology2000
UV-Induced stabilization of c-fos and other short-lived mRNAs.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 105 citations in OpenAlex.
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- Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with MitochondrialInternational journal of molecular sciences · 2023Article
- Roles and regulatory mechanisms of KIN17 in cancers (Review).Oncology letters · 2023Review
- Y box binding protein 1 inhibition as a targeted therapy for ovarian cancer.Cell chemical biology · 2021Article
- Reduced levels of prostaglandin IProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Connections between 3' end processing and DNA damage response: Ten years later.Wiley interdisciplinary reviews. RNA · 2020Review
- Targeting the upstream transcriptional activator of PD-L1 as an alternative strategy in melanoma therapy.Oncogene · 2018Article
- Stress and the nonsense-mediated RNA decay pathway.Cellular and molecular life sciences : CMLS · 2017Review
- Cellular responses to HSV-1 infection are linked to specific types of alterations in the host transcriptome.Scientific reports · 2016Article
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- Anoikis of colon carcinoma cells triggered by β-catenin loss can be enhanced by tumor necrosis factor receptor 1 antagonists.Oncogene · 2015Article
- Retroviral cyclin controls cyclin-dependent kinase 8-mediated transcription elongation and reinitiation.Journal of virology · 2015Article
- Expression of Kin17 promotes the proliferation of hepatocellular carcinoma cellsOncology letters · 2014Article
- Post-transcriptional regulation of DNA damage-responsive gene expression.Antioxidants & redox signaling · 2014Review
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- DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast.Genome research · 2013Article
- Transcriptional regulation of human DNA repair genes following genotoxic stress: trigger mechanisms, inducible responses and genotoxic adaptation.Nucleic acids research · 2013Review
- The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells.PloS one · 2013Article
- Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling.RNA biology · 2012Review
- Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals.Genome research · 2012Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Irradiation of cells with short-wavelength ultraviolet light (UVC) changes the program of gene expression, in part within less than 15 min. As one of the immediate-early genes in response to UV, expression of the oncogene c-fos is upregulated. This immediate induction is regulated at the transcriptional level and is transient in character, due to the autocatalyzed shutoff of transcription and the rapid turnover of c-fos mRNA. In an experiment analyzing the kinetics of c-fos mRNA expression in murine fibroblasts irradiated with UVC, we found that, in addition to the initial transient induction, c-fos mRNA accumulated in a second wave starting at 4 to 5 h after irradiation, reaching a maximum at 8 h, and persisting for several more hours. It was accompanied by an increase in Fos protein synthesis. The second peak of c-fos RNA was caused by an UV dose-dependent increase in mRNA half-life from about 10 to 60 min. With similar kinetics, the mRNAs of other UV target genes (i.e., the Kin17 gene, c-jun, IkappaB, and c-myc) were stabilized (e.g., Kin17 RNA from 80 min to more than 8 h). The delayed response was not due to autocrine cytokine secretion with subsequent autostimulation of the secreting cells or to UV-induced growth factor receptor activation. Cells unable to repair UVC-induced DNA damage responded to lower doses of UVC with an even greater accumulation of c-fos and Kin17 mRNAs than repair-proficient wild-type cells, suggesting that a process in which a repair protein is involved regulates mRNA stability. Although resembling the induction of p53, a DNA damage-dependent increase in p53 was not a necessary intermediate in the stabilization reaction, since cells derived from p53 knockout mice showed the same pattern of c-fos and Kin17 mRNA accumulation as wild-type cells. The data indicate that the signal flow induced by UV radiation addresses not only protein stability (p53) and transcription but also RNA stability, a hitherto-unrecognized level of UV-induced regulation.
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