ArticleMolecular and cellular biology2000
Phospholipase C is involved in kinetochore function in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Copper Accumulation Efficiency in Different Recombinant Microorganism Strains Available for Bioremediation of Heavy Metal-Polluted Waters.International journal of molecular sciences · 2023Article
- Lipids in the cell: organisation regulates function.Cellular and molecular life sciences : CMLS · 2018Review
- Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters.FEMS microbiology letters · 2017Article
- Yeast phospholipase C is required for normal acetyl-CoA homeostasis and global histone acetylation.The Journal of biological chemistry · 2013Article
- Inositol pyrophosphates modulate S phase progression after pheromone-induced arrest in Saccharomyces cerevisiae.The Journal of biological chemistry · 2013Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Roles of inositol phosphates and inositol pyrophosphates in development, cell signaling and nuclear processes.Advances in enzyme regulation · 2010Review
- Plc1p is required for proper chromatin structure and activity of the kinetochore in Saccharomyces cerevisiae by facilitating recruitment of the RSC complex.Molecular genetics and genomics : MGG · 2009Article
- Saccharomyces cerevisiae phospholipase C regulates transcription of Msn2p-dependent stress-responsive genes.Eukaryotic cell · 2008Article
- Nuclear phospholipid signaling: phosphatidylinositol-specific phospholipase C and phosphoinositide 3-kinase.Pflugers Archiv : European journal of physiology · 2007Review
- Plc1p is required for SAGA recruitment and derepression of Sko1p-regulated genes.Molecular biology of the cell · 2007Article
- Genetic evidence for a role of phospholipase C at the budding yeast kinetochore.Molecular genetics and genomics : MGG · 2003Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
The budding yeast PLC1 gene encodes a homolog of the delta isoform of mammalian phosphoinositide-specific phospholipase C. Here, we present evidence that Plc1p associates with the kinetochore complex CBF3. This association is mediated through interactions with two established kinetochore proteins, Ndc10p and Cep3p. We show by chromatin immunoprecipitation experiments that Plc1p resides at centromeric loci in vivo. Deletion of PLC1, as well as plc1 mutations which abrogate the interaction of Plc1p with the CBF3 complex, results in a higher frequency of minichromosome loss, nocodazole sensitivity, and mitotic delay. Overexpression of Ndc10p suppresses the nocodazole sensitivity of plc1 mutants, implying that the association of Plc1p with CBF3 is important for optimal kinetochore function. Chromatin extracts from plc1Delta cells exhibit reduced microtubule binding to minichromosomes. These results suggest that Plc1p associates with kinetochores and regulates some aspect of kinetochore function and demonstrate an intranuclear function of phospholipase C in eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.