ArticleMolecular and cellular biology2000
Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.Nucleic acids research · 2023Article
- Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes.Cell reports · 2017Article
- Article
- Role of the DHH1 gene in the regulation of monocarboxylic acids transporters expression in Saccharomyces cerevisiae.PloS one · 2014Article
- The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment.PloS one · 2014Article
- Structural integrity of centromeric chromatin and faithful chromosome segregation requires Pat1.Genetics · 2013Article
- The cellular decapping activators LSm1, Pat1, and Dhh1 control the ratio of subgenomic to genomic Flock House virus RNAs.Journal of virology · 2013Article
- Co-regulation of polar mRNA transport and lifespan in budding yeast Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2012Article
- Use of the novel technique of analytical ultracentrifugation with fluorescence detection system identifies a 77S monosomal translation complex.Protein science : a publication of the Protein Society · 2012Article
- Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins.Molecular cell · 2012Article
- RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.Molecular biology of the cell · 2012Article
- Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes.Nucleic acids research · 2011Article
- Distinct functions of maternal and somatic Pat1 protein paralogs.RNA (New York, N.Y.) · 2010Article
- HPat provides a link between deadenylation and decapping in metazoa.The Journal of cell biology · 2010Article
- Article
- Pat1 contains distinct functional domains that promote P-body assembly and activation of decapping.Molecular and cellular biology · 2008Article
- The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs.RNA (New York, N.Y.) · 2007Article
- The RNA polymerase II CTD kinase Ctk1 functions in translation elongation.Genes & development · 2007Article
- Host deadenylation-dependent mRNA decapping factors are required for a key step in brome mosaic virus RNA replication.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast poly(A) binding protein Pab1p mediates the interactions between the 5' cap structure and the 3' poly(A) tail of mRNA, whose structures synergistically activate translation in vivo and in vitro. We found that deletion of the PAT1 (YCR077c) gene suppresses a PAB1 gene deletion and that Pat1p is required for the normal initiation of translation. A fraction of Pat1p cosediments with free 40S ribosomal subunits on sucrose gradients. The PAT1 gene is not essential for viability, although disruption of the gene severely impairs translation initiation in vivo, resulting in the accumulation of 80S ribosomes and in a large decrease in the amounts of heavier polysomes. Pat1p contributes to the efficiency of translation in a yeast cell-free system. However, the synergy between the cap structure and the poly(A) tail is maintained in vitro in the absence of Pat1p. Analysis of translation initiation intermediates on gradients indicates that Pat1p acts at a step before or during the recruitment of the 40S ribosomal subunit by the mRNA, a step which may be independent of that involving Pab1p. We conclude that Pat1p is a new factor involved in protein synthesis and that Pat1p might be required for promoting the formation or the stabilization of the preinitiation translation complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.