ArticleBiophysical journal2000
Motion determination in actin filament fluorescence images with a spatio-temporal orientation analysis method.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Determination of the Maximum Velocity of Filaments in theFrontiers in physiology · 2019Article
- Extraction Protocols for Individual Zebrafish's Ventricle Myosin and Skeletal Muscle Actin forFrontiers in physiology · 2017Article
- The role of tropomyosin domains in cooperative activation of the actin-myosin interaction.Journal of molecular biology · 2011Article
- Actin-driven cell dynamics probed by Fourier transform light scattering.Biomedical optics express · 2010Article
- Myosin motors drive long range alignment of actin filaments.The Journal of biological chemistry · 2010Article
- Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single-molecule experiments.The Journal of physiology · 2006Article
- Computational imaging in cell biology.The Journal of cell biology · 2003Review
- Quantitative comparison of algorithms for tracking single fluorescent particles.Biophysical journal · 2001Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a novel approach of automatically measuring motion in series of microscopic fluorescence images. As a differential method, the three-dimensional structure tensor technique is used to calculate the displacement vector field for every image of the sequence, from which the velocities are subsequently derived. We have used this method for the analysis of the movement of single actin filaments in the in vitro motility assay, where fluorescently labeled actin filaments move over a myosin decorated surface. With its fast implementation and subpixel accuracy, this approach is, in general, very valuable for analyzing dynamic processes by image sequence analysis.
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Registered trials
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