ArticleBiophysical journal2000
Cell property determination from the acoustic microscope generated voltage versus frequency curves.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 85 citations in OpenAlex.
- Novel Water Probe for High-Frequency Focused Transducer Applied to Scanning Acoustic Microscopy System: Simulation and Experimental Investigation.Sensors (Basel, Switzerland) · 2024Article
- The Mechanics of Mitotic Cell Rounding.Frontiers in cell and developmental biology · 2020Review
- High resolution 3D imaging of living cells with sub-optical wavelength phonons.Scientific reports · 2016Article
- Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping.Nature communications · 2016Article
- Multi-layer phase analysis: quantifying the elastic properties of soft tissues and live cells with ultra-high-frequency scanning acoustic microscopy.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2012Article
- Localised micro-mechanical stiffening in the ageing aorta.Mechanisms of ageing and development · 2011Article
- Characterizing the elastic properties of tissues.Materials today (Kidlington, England) · 2011Article
- Quantifying Micro-mechanical Properties of Soft Biological Tissues with Scanning Acoustic Microscopy.Materials Research Society symposia proceedings. Materials Research Society · 2011Article
- Quantitative ultrasound backscatter for pulsed cavitational ultrasound therapy- histotripsy.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2009Article
- Mapping the Micromechanical Properties of Cryo-sectioned Aortic Tissue with Scanning Acoustic Microscopy.Materials Research Society symposia proceedings. Materials Research Society · 2009Article
- Subunits alpha, beta and gamma of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes.Pflugers Archiv : European journal of physiology · 2008Article
- Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence.Biophysical journal · 2005Article
- An acoustic microscopy technique reveals hidden morphological defenses in Daphnia.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the methods for the determination of mechanical properties of living cells acoustic microscopy provides some extraordinary advantages. It is relatively fast, of excellent spatial resolution and of minimal invasiveness. Sound velocity is a measure of the stiffness or Young's modulus of the cell. Attenuation of cytoplasm is a measure of supramolecular interactions. These parameters are of crucial interest for studies of cell motility, volume regulations and to establish the functional role of the various elements of the cytoskeleton. Using a phase and amplitude sensitive modulation of a scanning acoustic microscope (Hillman et al., 1994, J. Alloys Compounds. 211/212:625-627) longitudinal wave speed, attenuation and thickness profile of a biological cell are obtained from the voltage versus frequency or V(f) curves. A series of pictures, for instance in the frequency range 980-1100 MHz with an increment of 20 MHz, allows the experimental generation of V(f) curves for each pixel while keeping the lens-specimen distance unchanged. Both amplitude and phase values of the V(f) curves are used for obtaining the cell properties and the cell thickness profile. The theoretical analysis shows that the thin liquid layer, between the cell and the substrate, has a strong influence on the reflection coefficient and should not be ignored during the analysis. Cell properties, cell profile and the thickness of the thin liquid layer are obtained from the V(f) curves by the simplex inversion algorithm. The main advantages of this new method are that imaging can be done near the focal plane, therefore an optimal signal to noise ratio is achieved, no interference with Rayleigh waves occurs, and the method requires only an approximate estimate of the material properties of the solid substratum where the cells are growing on.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.