ArticleThe Journal of biological chemistry2000
Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha2(XI) collagen gene.
Article in The Journal of biological chemistry, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 94 citations in OpenAlex.
- The gene-regulatory evolution of the human skeleton.Nature · 2026Article
- Transcriptomic Profiling of Zebrafish Mutant forInternational journal of molecular sciences · 2025Article
- Association between adiponectin single nucleotide polymorphisms and the risk of diabetic polyneuropathy.Scientific reports · 2025Article
- Temporomandibular Joint Fibrocartilage Contains CD105 Positive Mouse Mesenchymal Stem/Progenitor Cells with Increased Chondrogenic Potential.Journal of maxillofacial and oral surgery · 2023Article
- Characterization of microRNA Levels in Synovial Fluid from Knee Osteoarthritis and Anterior Cruciate Ligament Tears.Biomedicines · 2022Article
- Dominant-negative SOX9 mutations in campomelic dysplasia.Human mutation · 2019Article
- Dichotomy in redundant enhancers points to presence of initiators of gene regulation.BMC genomics · 2018Article
- Association of Adiponectin and rs1501299 of theBiomolecules · 2018Article
- Transcriptional Network Controlling Endochondral Ossification.Journal of bone metabolism · 2017Review
- The Potential of microRNAs for Stem Cell-based Therapy for Degenerative Skeletal Diseases.Current molecular biology reports · 2017Review
- Sp1 upregulates the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes.In vitro cellular & developmental biology. Animal · 2016Article
- Distinct Transcriptional Programs Underlie Sox9 Regulation of the Mammalian Chondrocyte.Cell reports · 2015Article
- On the evolutionary relationship between chondrocytes and osteoblasts.Frontiers in genetics · 2015Review
- Cav3.2 T-type calcium channel is required for the NFAT-dependent Sox9 expression in tracheal cartilage.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Nuclear factor Y (NF-Y) regulates the proximal promoter activity of the mouse collagen α1(XI) gene (Col11a1) in chondrocytes.In vitro cellular & developmental biology. Animal · 2014Article
- How to build an inducible cartilage-specific transgenic mouse.Arthritis research & therapy · 2014Review
- Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors.PloS one · 2013Article
- Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification.The Journal of biological chemistry · 2012Article
- Notch1 signaling regulates chondrogenic lineage determination through Sox9 activation.Cell death and differentiation · 2012Article
- SOX9 governs differentiation stage-specific gene expression in growth plate chondrocytes via direct concomitant transactivation and repression.PLoS genetics · 2011Article
Corrections and comments
- Commented on by
- Erratum issued
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type XI collagen, a heterotrimer composed of alpha1(XI), alpha2(XI) and alpha3(XI), is primarily synthesized by chondrocytes in cartilage and is also present in some other tissues. Type XI collagen plays a critical role in collagen fibril formation and skeletal morphogenesis. We investigated a tissue-specific transcriptional enhancer in the first intron of the alpha2(XI) collagen gene (Col11a2). Transient transfection assays using reporter gene constructs revealed that a 60-base pair (bp) segment within intron 1 increased promoter activity of Col11a2 in rat chondrosarcoma cells but not in either BalB/3T3 cells or undifferentiated ATDC5 cells, suggesting that it contained cell type-specific enhancer activity. In transgenic mice, this 60-bp fragment was also able to target beta-galactosidase expression to cartilage including the limbs and axial skeleton, with similar localization specificity as the full-length intron 1 fragment. Competition experiments in gel shift assays using mutated oligonucleotides showed that recombinant Sox9 bound to a 7-bp sequence, CTCAAAG, within the 60-bp segment. Anti-Sox9 antibodies supershifted the complex of the 60-bp segment with recombinant Sox9 or with rat chondrosarcoma cell extracts, confirming the binding of Sox9 to the enhancer. Moreover, a site-specific mutation within the 7-bp segment resulted in essentially complete loss of the enhancer activity in chondrosarcoma cells and transgenic mice. These results suggest that the 7-bp sequence within intron 1 plays a critical role in the cartilage-specific enhancer activity of Col11a2 through Sox9-mediated transcriptional activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.