ArticleJournal of virology2000
Cytoplasmic trafficking of the canine parvovirus capsid and its role in infection and nuclear transport.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
- Integrative Analysis of Dog Serum-Derived CircRNA Expression and Disease Severity, Inflammatory and Cardiac Damage Biomarkers Related to Canine Parvoviral Enteritis.Veterinary medicine and science · 2025Article
- Concepts to Reveal Parvovirus-Nucleus Interactions.Viruses · 2021Review
- Viral highway to nucleus exposed by image correlation analyses.Scientific reports · 2018Article
- Protoparvovirus Cell Entry.Viruses · 2017Review
- Protoparvovirus Knocking at the Nuclear Door.Viruses · 2017Review
- Parvovirus Capsid Structures Required for Infection: Mutations Controlling Receptor Recognition and Protease Cleavages.Journal of virology · 2017Article
- Coat as a dagger: the use of capsid proteins to perforate membranes during non-enveloped DNA viruses trafficking.Viruses · 2014Review
- Parvovirus particles and movement in the cellular cytoplasm and effects of the cytoskeleton.Virology · 2014Article
- Parvoviruses cause nuclear envelope breakdown by activating key enzymes of mitosis.PLoS pathogens · 2013Article
- The potential role of microfilaments in host cells for infection with infectious spleen and kidney necrosis virus infection.Virology journal · 2013Article
- Characterization of the early steps of human parvovirus B19 infection.Journal of virology · 2012Article
- Mutations at the base of the icosahedral five-fold cylinders of minute virus of mice induce 3'-to-5' genome uncoating and critically impair entry functions.Journal of virology · 2012Article
- The combined effects of oncolytic reovirus plus Newcastle disease virus and reovirus plus parvovirus on U87 and U373 cells in vitro and in vivo.Journal of neuro-oncology · 2011Article
- Multiple pathways involved in porcine parvovirus cellular entry and trafficking toward the nucleus.Journal of virology · 2010Article
- Densovirus infectious pathway requires clathrin-mediated endocytosis followed by trafficking to the nucleus.Journal of virology · 2009Article
- Effect of inhibition of dynein function and microtubule-altering drugs on AAV2 transduction.Virology · 2007Article
- Intracellular transport of hepatitis B virus.World journal of gastroenterology · 2007Review
- Adeno-associated virus type 2 capsids with externalized VP1/VP2 trafficking domains are generated prior to passage through the cytoplasm and are maintained until uncoating occurs in the nucleus.Journal of virology · 2006Article
- Intracellular trafficking of plasmids during transfection is mediated by microtubules.Molecular therapy : the journal of the American Society of Gene Therapy · 2006Article
- VP2 cleavage and the leucine ring at the base of the fivefold cylinder control pH-dependent externalization of both the VP1 N terminus and the genome of minute virus of mice.Journal of virology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To begin a successful infection, viruses must first cross the host cell plasma membrane, either by direct fusion with the membrane or by receptor-mediated endocytosis. After release into the cytoplasm those viruses that replicate in the nucleus must target their genome to that location. We examined the role of cytoplasmic transport of the canine parvovirus (CPV) capsid in productive infection by microinjecting two antibodies that recognize the intact CPV capsid into the cytoplasm of cells and also by using intracellular expression of variable domains of a neutralizing antibody fused to green fluorescence protein. The two antibodies tested and the expressed scFv all efficiently blocked virus infection, probably by binding to virus particles while they were in the cytoplasm and before entering the nucleus. The injected antibodies were able to block most infections even when injected 8 h after virus inoculation. In control studies, microinjected capsid antibodies did not interfere with CPV replication when they were coinjected with an infectious plasmid clone of CPV. Cytoplasmically injected full and empty capsids were able to move through the cytosol towards the nuclear membrane in a process that could be blocked by nocodazole treatment of the cells. Nuclear transport of the capsids was slow, with significant amounts being found in the nucleus only 3 to 6 h after injection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.