ArticleThe EMBO journal2000
Nek2B, a novel maternal form of Nek2 kinase, is essential for the assembly or maintenance of centrosomes in early Xenopus embryos.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 89 citations in OpenAlex.
- Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics.eLife · 2025Article
- Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction.The Journal of cell biology · 2020Article
- Prolonged ovarian storage of matureeLife · 2019Article
- Nek2B activates the wnt pathway and promotes triple-negative breast cancer chemothezrapy-resistance by stabilizing β-catenin.Journal of experimental & clinical cancer research : CR · 2019Article
- Translational activation of maternally derived mRNAs in oocytes and early embryos and the role of embryonic poly(A) binding protein (EPAB).Biology of reproduction · 2019Review
- Prognostic value of NEK2 overexpression in digestive system cancers: a meta-analysis and systematic review.OncoTargets and therapy · 2018Article
- NIMA-related kinase 2 regulates hepatocellular carcinoma cell growth and proliferation.Oncology letters · 2017Article
- Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses.Nature communications · 2017Article
- Effect of silencing NEK2 on biological behaviors of HepG2 in human hepatoma cells and MAPK signal pathway.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Overexpression of NIMA-related kinase 2 is associated with progression and poor prognosis of prostate cancer.BMC urology · 2015Article
- Nek2 is a novel regulator of B cell development and immunological response.BioMed research international · 2014Article
- Identification and characterization of human embryonic poly(A) binding protein (EPAB).Molecular human reproduction · 2008Article
- Isolation of the human ePAB and ePABP2 cDNAs and analysis of the expression patterns.Journal of assisted reproduction and genetics · 2008Article
- Mitotic regulation by NIMA-related kinases.Cell division · 2007Article
- Dynamic recruitment of Nek2 kinase to the centrosome involves microtubules, PCM-1, and localized proteasomal degradation.Molecular biology of the cell · 2005Article
- An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.Molecular biology of the cell · 2004Article
- TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest.Molecular biology of the cell · 2003Article
- Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles.Molecular biology of the cell · 2003Article
- Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes.The Journal of cell biology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nek2, a NIMA-related kinase, has been postulated to play a role in both the meiotic and mitotic cell cycles in vertebrates. Xenopus has two Nek2 splice variants, Nek2A and Nek2B, which are zygotic and maternal forms, respectively. Here we have examined the role of Nek2B in oocyte meiosis and early embryonic mitosis. Specific inhibition of Nek2B function does not interfere with the oscillation of Cdc2 activity in either the meiotic or mitotic cell cycles; however, it does cause abortive cleavage of early embryos, in which bipolar spindle formation is severely impaired due to fragmentation or dispersal of the centrosomes, to which endogenous Nek2B protein localizes. In contrast, inhibition of Nek2B function does not affect meiotic spindle formation in oocytes, in which functional centrosomes are absent. Thus, strikingly, Nek2B is specifically required for centrosome assembly and/or maintenance (and hence for normal bipolar spindle formation and cleavage) in early Xenopus embryos. Finally, (ectopic) Nek2A but not Nek2B is very labile in cleaving embryos, suggesting that Nek2A cannot replace the centrosomal function of Nek2B in early embryos.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.