ArticleNucleic acids research2000
The complete DNA sequence and analysis of R27, a large IncHI plasmid from Salmonella typhi that is temperature sensitive for transfer.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 173 citations in OpenAlex.
- Solution characterization of TrhW, a type 4 secretion system regulatory protein from the R27 plasmid.Structural dynamics (Melville, N.Y.) · 2026Article
- Stable inheritance of the Streptomyces linear plasmid SCP1 by dual ParABS partition systems.Nucleic acids research · 2026Article
- Genomic Epidemiology of FoodborneAntibiotics (Basel, Switzerland) · 2026Article
- Genomic characterization of ceftazidime/avibactam-resistantFrontiers in microbiology · 2026Article
- Plasmid copy number as a modulator in bacterial pathogenesis and antibiotic resistance.npj antimicrobials and resistance · 2025Review
- Whole genome sequence analysis of multidrug-resistant Salmonella enterica Typhimurium ms203 provides insights into virulence and antibiotic resistance.Current genetics · 2025Article
- Genomic characterization of extended-spectrum β-lactamase-producingApplied and environmental microbiology · 2025Article
- Mechanisms of Transmission and Adaptation ofVeterinary sciences · 2025Article
- Cryo-EM structure of the conjugation H-pilus reveals the cyclic nature of the TrhA pilin.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The pathway to resolve dimeric forms distinguishes plasmids from megaplasmids in Enterobacteriaceae.Nucleic acids research · 2025Article
- Sequencing Analysis of Invasive Carbapenem-ResistantMicroorganisms · 2025Article
- Identification of an IncHI5-like plasmid co-harboringFrontiers in microbiology · 2025Article
- Article
- The association between the genetic structures of commonly incompatible plasmids in Gram-negative bacteria, their distribution and the resistance genes.Frontiers in cellular and infection microbiology · 2024Review
- An exploration of unusual antimicrobial resistance phenotypes in Salmonella Typhi from Blantyre, Malawi reveals the ongoing role of IncHI1 plasmids.Gates open research · 2024Article
- Article
- The potential application of carbazole-degrading bacteria for dioxin bioremediation.Bioresources and bioprocessing · 2023Review
- Genome-Based Epidemiologic Analysis of VIM/IMP Carbapenemase-Producing Enterobacter spp., Poland.Emerging infectious diseases · 2023Article
- Genomic characterization of Salmonella enterica serovar Choleraesuis from Brazil reveals a swine gallbladder isolate harboring colistin resistance gene mcr-1.1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Conjugative Transfer of Acute Hepatopancreatic Necrosis Disease-Causing pVA1-Type Plasmid Is Mediated by a Novel Self-Encoded Type IV Secretion System.Microbiology spectrum · 2022Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Salmonella typhi, the causative agent of typhoid fever, annually infects 16 million people and kills 600 000 world wide. Plasmid-encoded multiple drug resistance in S. typhi is always encoded by plasmids of incompatibility group H (IncH). The complete DNA sequence of the large temperature-sensitive conjugative plasmid R27, the prototype for the IncHI1 family of plasmids, has been compiled and analyzed. This 180 kb plasmid contains 210 open reading frames (ORFs), of which 14 have been previously identified and 56 exhibit similarity to other plasmid and prokaryotic ORFs. A number of insertion elements were found, including the full Tn 10 transposon, which carries tetracycline resistance genes. Two transfer regions, Tra1 and Tra2, are present, which are separated by a minimum of 64 kb. Homologs of the DNA-binding proteins TlpA and H-NS that act as temperature-regulated repressors in other systems have been located in R27. Sequence analysis of transfer and replication regions supports a mosaic-like structure for R27. The genes responsible for conjugation and plasmid maintenance have been identified and mechanisms responsible for thermosensitive transfer are discussed.
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