ArticleNucleic acids research2000
Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Impact of guanidine-containing backbone linkages on stereopure antisense oligonucleotides in the CNS.Nucleic acids research · 2022Article
- Control of backbone chemistry and chirality boost oligonucleotide splice switching activity.Nucleic acids research · 2022Article
- The ABCF gene family facilitates disaggregation during animal development.Molecular biology of the cell · 2020Review
- Evolution and development of the tetrapod auditory system: an organ of Corti-centric perspective.Evolution & development · 2013Review
- Synthetic strategies for studying embryonic development.Chemistry & biology · 2010Review
- The use of antisense oligonucleotides in Xenopus oocytes.Methods (San Diego, Calif.) · 2010Review
- EYA1 mutations associated with the branchio-oto-renal syndrome result in defective otic development in Xenopus laevis.Biology of the cell · 2010Article
- Hoogsteen-paired homopurine [RP-PS]-DNA and homopyrimidine RNA strands form a thermally stable parallel duplex.Biophysical journal · 2007Article
- TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation.The EMBO journal · 2007Article
- Unusual thermal stability of RNA/[RP-PS]-DNA/RNA triplexes containing a homopurine DNA strand.Biophysical journal · 2007Article
- Chordin affects pronephros development in Xenopus embryos by anteriorizing presomitic mesoderm.Developmental dynamics : an official publication of the American Association of Anatomists · 2007Article
- Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Cationic phosphoramidate alpha-oligonucleotides efficiently target single-stranded DNA and RNA and inhibit hepatitis C virus IRES-mediated translation.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have designed a new class of modified antisense oligodeoxyribonucleotides (ODN) consisting of a central contiguous stretch of 6-8 unmodified nucleotides flanked by 3'- and 5'-regions containing several nucleotides joined by cationic internucleoside linkages. The positive charge results from modification of the internucleoside linkages as N, N -diethylethylene-diamine phosphoramidates. These zwitterionic compounds show improved antisense activity in both Xenopus oocytes and embryos compared to our previously described chimeric oligonucleotides possessing neutral terminal internucleoside linkages. Using the localized maternal mRNA An2 as a target, we have shown that chimeric oligonucleotides with terminal positive charges are very effective in the sequence-specific elimination of maternal messages present in both oocytes and embryos. In addition, using the embryonic mRNA GS17 as a target, we have shown that these oligonucleotides can direct RNase H-mediated cleavage of messages produced at the onset of zygotic transcription, after the mid-blastula stage. These new compounds should be useful in attenuating embryonic gene expression to study the role of specific proteins in early vertebrate development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.