ArticleNucleic acids research2000
Bi-directional replication and random termination.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms.Nucleic acids research · 2025Article
- Concise Overview of Methodologies Employed in the Study of Bacterial DNA Replication.International journal of molecular sciences · 2025Review
- Control of DNA replication in vitro using a reversible replication barrier.Nature protocols · 2024Article
- The abundance of Fob1 modulates the efficiency of rRFBs to stall replication forks.Nucleic acids research · 2017Article
- Replication landscape of the human genome.Nature communications · 2016Article
- Article
- Termination of DNA replication forks: "Breaking up is hard to do".Nucleus (Austin, Tex.) · 2015Article
- Topoisomerase 2 is dispensable for the replication and segregation of small yeast artificial chromosomes (YACs).PloS one · 2014Article
- Replicating damaged DNA in eukaryotes.Cold Spring Harbor perspectives in biology · 2013Review
- Effects of Friedreich's ataxia GAA repeats on DNA replication in mammalian cells.Nucleic acids research · 2012Article
- Asymmetry indices for analysis and prediction of replication origins in eukaryotic genomes.PloS one · 2012Article
- Article
- Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea.Journal of bacteriology · 2008Article
- Molecular characterization of the minimal replicon and the unidirectional theta replication of pSCM201 in extremely halophilic archaea.Journal of bacteriology · 2006Article
- Replication-associated strand asymmetries in mammalian genomes: toward detection of replication origins.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Rad52-independent accumulation of joint circular minichromosomes during S phase in Saccharomyces cerevisiae.Molecular and cellular biology · 2003Article
- Complex mechanism of site-specific DNA replication termination in fission yeast.The EMBO journal · 2003Article
- A DNA replication-arrest site RTS1 regulates imprinting by determining the direction of replication at mat1 in S. pombe.Genes & development · 2001Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-dimensional (2D) agarose gel electrophoresis was used to study termination of DNA replication in a shuttle vector, YRp7', when it replicated in Escherichia coli, Saccharomyces cerevisiae and Xenopus egg extracts. In E. coli, the 2D gel patterns obtained were consistent with uni-directional replication initiated at a specific site, the ColE1 origin. In consequence, termination also occurred precisely at the ColE1 origin. In Xenopus egg extracts, the particular shape of the bubble arc as well as the triangular smear detected to the left of the simple-Y pattern indicated random initiation and termination. In S.cerevisiae, initiation occurred at the ARS1 origin and replication proceeded in a bi-directional manner. However, termination did not always occur at a specific site 180 degrees across from the origin, but almost all along the south hemisphere of the plasmid. Inversion, deletion or replacement of DNA sequences located throughout this hemisphere did not eliminate random termination. Analysis of the replication intermediates of another yeast plasmid bearing a different origin, ARS305, also exhibited random termination. We propose that the random termination events observed in S.cerevisiae could be due to an asynchronous departure of both forks from the bi-directional origin in addition to differences in the rate of fork progression. These observations could be extended to all bi-directional origins.
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