ArticleNucleic acids research2000
Optimizing heterologous expression in dictyostelium: importance of 5' codon adaptation.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- Expression of blue pigment synthetase a from Streptomyces lavenduale reveals insights on the effects of refactoring biosynthetic megasynthases for heterologous expression in Escherichia coli.Protein expression and purification · 2023Article
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- Allorecognition, via TgrB1 and TgrC1, mediates the transition from unicellularity to multicellularity in the social amoeba Dictyostelium discoideum.Development (Cambridge, England) · 2015Article
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- Codon-optimized antibiotic resistance gene improves efficiency of transient transformation in Frankia.Journal of biosciences · 2013Article
- A comparative analysis on the synonymous codon usage pattern in viral functional genes and their translational initiation region of ASFV.Virus genes · 2013Article
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- The distribution of synonymous codon choice in the translation initiation region of dengue virus.PloS one · 2013Article
- Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression.PloS one · 2011Article
- The GeneOptimizer Algorithm: using a sliding window approach to cope with the vast sequence space in multiparameter DNA sequence optimization.Systems and synthetic biology · 2010Article
- Lactococcus lactis, an alternative system for functional expression of peripheral and intrinsic Arabidopsis membrane proteins.PloS one · 2010Article
- Functional characterization of intracellular Dictyostelium discoideum P2X receptors.The Journal of biological chemistry · 2009Article
- Expression of plant flavor genes in Lactococcus lactis.Applied and environmental microbiology · 2007Article
- Comparative analysis of expressed sequences reveals a conserved pattern of optimal codon usage in plants.Plant molecular biology · 2006Article
- Reevaluation of the predicted gene structure of Dictyostelium cystatin A3 (cpiC) by nucleotide sequence determination of its cDNA* and its phylogenetic position in the cystatin superfamily.Molecular biology reports · 2005Article
- Attenuated Salmonella enterica serovar Typhi and Shigella flexneri 2a strains mucosally deliver DNA vaccines encoding measles virus hemagglutinin, inducing specific immune responses and protection in cotton rats.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of heterologous proteins in Dictyostelium discoideum presents unique research opportunities, such as the functional analysis of complex human glycoproteins after random mutagenesis. In one study, human chorionic gonadotropin (hCG) and human follicle stimulating hormone were expressed in Dictyostelium. During the course of these experiments, we also investigated the role of codon usage and of the DNA sequence upstream of the ATG start codon. The Dictyostelium genome has a higher AT content than the human, resulting in a different codon preference. The hCG-beta gene contains three clusters with infrequently used codons that were changed to codons that are preferred by Dictyostelium. The results reported here show that optimizing the first 5-17 codons of the hCG gene contributes to 4- to 5-fold increased expression levels, but that further optimization has no significant effect. These observations suggest that optimal codon usage contributes to ribosome stabilization, but does not play an important role during the elongation phase of translation. Furthermore, adapting the 5'-sequence of the hCG gene to the Dictyostelium 'Kozak'-like sequence increased expression levels approximately 1.5-fold. Thus, using both codon optimization and 'Kozak' adaptation, a 6- to 8-fold increase in expression levels could be obtained for hCG.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.