ArticleNucleic acids research2000
Encephalitozoon cuniculi (Microspora) genome: physical map and evidence for telomere-associated rDNA units on all chromosomes.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 64 citations in OpenAlex.
- Variation in Genome Architecture and Epigenetic Modification Across the Microsporidia Phylogeny.Genome biology and evolution · 2025Article
- Telomere-to-Telomere genome assemblies of human-infecting Encephalitozoon species.BMC genomics · 2023Article
- Impact of Genome Reduction in Microsporidia.Experientia supplementum (2012) · 2022Article
- Comparative Genomics of Microsporidia.Experientia supplementum (2012) · 2022Article
- Simplified Transformation ofGenes · 2019Article
- Microsporidia: Obligate Intracellular Pathogens Within the Fungal Kingdom.Microbiology spectrum · 2017Review
- Subtelomere organization in the genome of the microsporidian Encephalitozoon cuniculi: patterns of repeated sequences and physicochemical signatures.BMC genomics · 2016Article
- Extremely reduced levels of heterozygosity in the vertebrate pathogen Encephalitozoon cuniculi.Eukaryotic cell · 2013Article
- Characterization of active ribosomal RNA harboring MITEs insertion in microsporidian Nosema bombycis genome.Parasitology research · 2013Article
- Why the International Workshops on Opportunistic Protists?Eukaryotic cell · 2009Article
- Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi.PLoS pathogens · 2009Article
- Stripped-down DNA repair in a highly reduced parasite.BMC molecular biology · 2007Article
- Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora).Genome research · 2001Article
- In-gel DNA radiolabelling and two-dimensional pulsed field gel electrophoresis procedures suitable for fingerprinting and mapping small eukaryotic genomes.Nucleic acids research · 2000Article
- Minimization and complete loss of general transcription factor proteins in the intracellular parasiteTranscriptionArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A restriction map of the 2.8-Mb genome of the unicellular eukaryote Encephalitozoon cuniculi (phylum Microspora), a mammal-infecting intracellular parasite, has been constructed using two restriction enzymes with 6 bp recognition sites (Bss HII and Mlu I). The fragments resulting from either single digestions of the whole molecular karyotype or double digestions of 11 individual chromosomes have been separated by two-dimensional pulsed field gel electrophoresis (2D-PFGE) procedures. The average distance between successive restriction sites is approximately 19 kb. The terminal regions of the chromosomes show a common pattern covering approximately 15 kb and including one 16S-23S rDNA unit. Results of hybridisation and molecular combing experiments indicate a palindromic-like orientation of the two subtelomeric rDNA copies on each chromosome. We have also located 67 DNA markers (clones from a partial E. cuniculi genomic library) by hybridisation to restriction fragments. Partial or complete sequencing has revealed homologies with known protein-coding genes for 32 of these clones. Evidence for two homologous chromosomes III, with a size difference (3 kb) related to a subtelomeric deletion/insertion event, argues for diploidy of E.cuniculi. The physical map should be useful for both the whole genome sequencing project and studies on genome plasticity of this widespread parasite.
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