ArticleThe Journal of clinical investigation2000
Novel amiloride-sensitive sodium-dependent proton secretion in the mouse proximal convoluted tubule.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 115 citations in OpenAlex.
- The Physiological Function and Potential Role of the Ubiquitous NaInternational journal of molecular sciences · 2022Review
- The Role of Plasma Membrane Sodium/Hydrogen Exchangers in Gastrointestinal Functions: Proliferation and Differentiation, Fluid/Electrolyte Transport and Barrier Integrity.Frontiers in physiology · 2022Review
- A role for tubular NaAmerican journal of physiology. Renal physiology · 2020Article
- Sex differences in solute transport along the nephrons: effects of NaAmerican journal of physiology. Renal physiology · 2020Article
- Dopamine reduces cell surface NaAmerican journal of physiology. Renal physiology · 2017Article
- Light-dependent expression of a NaPhysiological reports · 2017Article
- NaFrontiers in physiology · 2017Article
- Solute transport and oxygen consumption along the nephrons: effects of Na+ transport inhibitors.American journal of physiology. Renal physiology · 2016Article
- Developmental changes in renal tubular transport-an overview.Pediatric nephrology (Berlin, Germany) · 2015Review
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Na+-H+ exchanger-1 (NHE1) regulation in kidney proximal tubule.Cellular and molecular life sciences : CMLS · 2015Review
- Renal NHE expression and activity in neonatal NHE3- and NHE8-null mice.American journal of physiology. Renal physiology · 2015Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Molecular mechanisms of renal and extrarenal manifestations caused by inactivation of the electrogenic Na(+)-HCO3 (-) cotransporter NBCe1.Frontiers in physiology · 2013Review
- Proximal tubular NHEs: sodium, protons and calcium?American journal of physiology. Renal physiology · 2013Review
- Proximal tubule Na+/H+ exchanger activity in adult NHE8-/-, NHE3-/-, and NHE3-/-/NHE8-/- mice.American journal of physiology. Renal physiology · 2012Article
- Acid increases NHE8 surface expression and activity in NRK cells.American journal of physiology. Renal physiology · 2012Article
- Expression of metanephric nephron-patterning genes in differentiating mesonephric tubules.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
- Effect of metabolic acidosis on neonatal proximal tubule acidification.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Mechanisms of the regulation of the intestinal Na+/H+ exchanger NHE3.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
The proximal convoluted tubule (PCT) reabsorbs most of the filtered bicarbonate. Proton secretion is believed to be mediated predominantly by an apical membrane Na(+)/H(+) exchanger (NHE). Several NHE isoforms have been cloned, but only NHE3 and NHE2 are known to be present on the apical membrane of the PCT. Here we examined apical membrane PCT sodium-dependent proton secretion of wild-type (NHE3(+/+)/NHE2(+/+)), NHE3(-/-), NHE2(-/-), and double-knockout NHE3(-/-)/NHE2(-/-) mice to determine their relative contribution to luminal proton secretion. NHE2(-/-) and wild-type mice had comparable rates of sodium-dependent proton secretion. Sodium-dependent proton secretion in NHE3(-/-) mice was approximately 50% that of wild-type mice. The residual sodium-dependent proton secretion was inhibited by 100 microM 5-(N-ethyl-N-isopropyl) amiloride (EIPA). Luminal sodium-dependent proton secretion was the same in NHE3(-/-)/NHE2(-/-) as in NHE3(-/-) mice. These data point to a previously unrecognized Na(+)-dependent EIPA-sensitive proton secretory mechanism in the proximal tubule that may play an important role in acid-base homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.