ArticleAntimicrobial agents and chemotherapy2000
Identification and expression of genes involved in biosynthesis of L-oleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus.
Article in Antimicrobial agents and chemotherapy, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 102 citations in OpenAlex.
- Engineering BioBricks for Deoxysugar Biosynthesis and Generation of New Tetracenomycins.ACS omega · 2023Article
- Dactylosporolides: Glycosylated Macrolides fromJournal of natural products · 2022Article
- De novo biosynthesis of garbanzol and fustin in Streptomyces albus based on a potential flavanone 3-hydroxylase with 2-hydroxylase side activity.Microbial biotechnology · 2021Article
- Crossroads of Antibiotic Resistance and Biosynthesis.Journal of molecular biology · 2019Review
- New Insights into the Biosynthesis Pathway of Polyketide Alkaloid Argimycins P inFrontiers in microbiology · 2018Article
- De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor.PloS one · 2018Article
- Engineering Heterologous Production of Salicylate Glucoside and Glycosylated Variants.Frontiers in microbiology · 2018Article
- Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water.PloS one · 2018Article
- Article
- Identification by Genome Mining of a Type I Polyketide Gene Cluster fromFrontiers in microbiology · 2017Article
- Increasing antibiotic production yields by favoring the biosynthesis of precursor metabolites glucose-1-phosphate and/or malonyl-CoA in Streptomyces producer strains.The Journal of antibiotics · 2016Article
- Tailoring pathway modularity in the biosynthesis of erythromycin analogs heterologously engineered in E. coli.Science advances · 2015Article
- Steps towards the synthetic biology of polyketide biosynthesis.FEMS microbiology letters · 2014Review
- Engineered biosynthesis of disaccharide-modified polyene macrolides.Applied and environmental microbiology · 2013Article
- Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin.Angewandte Chemie (International ed. in English) · 2012Article
- Investigating Mithramycin deoxysugar biosynthesis: enzymatic total synthesis of TDP-D-olivose.Chembiochem : a European journal of chemical biology · 2011Article
- Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics.Chembiochem : a European journal of chemical biology · 2009Article
- SpnH from Saccharopolyspora spinosa encodes a rhamnosyl 4'-O-methyltransferase for biosynthesis of the insecticidal macrolide, spinosyn A.Journal of industrial microbiology & biotechnology · 2008Article
- Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose.The Biochemical journal · 2008Article
- Natural-product sugar biosynthesis and enzymatic glycodiversification.Angewandte Chemie (International ed. in English) · 2008Review
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 9.8-kb DNA region from the oleandomycin gene cluster in Streptomyces antibioticus was cloned. Sequence analysis revealed the presence of 8 open reading frames encoding different enzyme activities involved in the biosynthesis of one of the two 2, 6-deoxysugars attached to the oleandomycin aglycone: L-oleandrose (the oleW, oleV, oleL, and oleU genes) and D-desosamine (the oleNI and oleT genes), or of both (the oleS and oleE genes). A Streptomyces albus strain harboring the oleG2 glycosyltransferase gene integrated into the chromosome was constructed. This strain was transformed with two different plasmid constructs (pOLV and pOLE) containing a set of genes proposed to be required for the biosynthesis of dTDP-L-olivose and dTDP-L-oleandrose, respectively. Incubation of these recombinant strains with the erythromycin aglycon (erythronolide B) gave rise to two new glycosylated compounds, identified as L-3-O-olivosyl- and L-3-O-oleandrosyl-erythronolide B, indicating that pOLV and pOLE encode all enzyme activities required for the biosynthesis of these two 2,6-dideoxysugars. A pathway is proposed for the biosynthesis of these two deoxysugars in S. antibioticus.
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