Evidence map›Paper›PMID 10769178›Full record

ArticleThe Biochemical journal2000

Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi.

I Mouyna, M Monod, T Fontaine, B Henrissat, B Léchenne, J P Latgé

Open access · greenAbstract read
In one paragraph

Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 73 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019
    Review
  5. Cell wall glucans of fungi. A review.Cell surface (Amsterdam, Netherlands) · 2019
    Review
  6. Review
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  18. Article
  19. Molecular mechanisms of yeast cell wall glucan remodeling.The Journal of biological chemistry · 2009
    Article
  20. Cell wall assembly in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

I MouynaInstitut Pasteur, Laboratoire des Aspergillus, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. imouyna@pasteur.fr
M Monod
T Fontaine
B Henrissat
B Léchenne
J P Latgé
Institut Pasteur · FRHôpital de Beaumont · CHCentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A new family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional similarity to the Gas/Phr/Epd protein families, which are involved in yeast morphogenesis. Sequence comparisons and hydrophobic cluster analysis (HCA) showed that all the Gas/Phr/Epd/Gel proteins belong to a new family of glycosylhydrolases, family 72. We confirmed by site-directed mutagenesis and biochemical analysis that the two conserved glutamate residues (the putative catalytic residues of this family, as determined by HCA) are involved in the active site of this family of glycosylhydrolases.

Indexed as

Catalytic DomainAmino Acid SequenceAspergillus fumigatusBinding SitesBlotting, WesternConserved SequenceFungal ProteinsGlucan Endo-1,3-beta-D-GlucosidaseGlutamic AcidGlycosylphosphatidylinositolsMolecular Sequence DataMultigene FamilyMutationProtein Structure, SecondaryRecombinant ProteinsSequence Alignment1,3-beta-glucanosyltransferaseFungal ProteinsGlucan Endo-1,3-beta-D-GlucosidaseGlutamic AcidGlycosylphosphatidylinositolsRecombinant Proteins

Identifiers

PMID10769178
PMCPMC1221011
OpenAlexW1965468221

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.