ArticleThe Journal of cell biology2000
MEK and Cdc2 kinase are sequentially required for Golgi disassembly in MDCK cells by the mitotic Xenopus extracts.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 90 citations in OpenAlex.
- Establishment and phenotyping of disease model cells created by cell-resealing technique.Scientific reports · 2017Article
- Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis.Molecular biology of the cell · 2017Article
- Golgi structure formation, function, and post-translational modifications in mammalian cells.F1000Research · 2017Review
- PKD controls mitotic Golgi complex fragmentation through a Raf-MEK1 pathway.Molecular biology of the cell · 2013Article
- MEK1 inactivates Myt1 to regulate Golgi membrane fragmentation and mitotic entry in mammalian cells.The EMBO journal · 2013Article
- Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.Molecular biology of the cell · 2012Article
- A resealed-cell system for analyzing pathogenic intracellular events: perturbation of endocytic pathways under diabetic conditions.PloS one · 2012Article
- Golgi biogenesis.Cold Spring Harbor perspectives in biology · 2011Review
- Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand.The Journal of biological chemistry · 2010Article
- Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free system.Nature protocols · 2010Article
- Specific phosphorylation and activation of ERK1c by MEK1b: a unique route in the ERK cascade.Genes & development · 2009Article
- Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.Molecular and cellular biology · 2009Article
- GRASP55 regulates Golgi ribbon formation.Molecular biology of the cell · 2008Article
- The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis.Molecular biology of the cell · 2008Article
- Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay.The Journal of biological chemistry · 2008Article
- Active ADP-ribosylation factor-1 (ARF1) is required for mitotic Golgi fragmentation.The Journal of biological chemistry · 2007Article
- Mitogen-activated protein kinase kinase 1-dependent Golgi unlinking occurs in G2 phase and promotes the G2/M cell cycle transition.Molecular biology of the cell · 2007Article
- The Golgi-associated protein GRASP65 regulates spindle dynamics and is essential for cell division.Molecular biology of the cell · 2005Article
- Cdc2 kinase-dependent disassembly of endoplasmic reticulum (ER) exit sites inhibits ER-to-Golgi vesicular transport during mitosis.Molecular biology of the cell · 2004Article
- MEK1-induced Golgi dynamics during cell cycle progression is partly mediated by Polo-like kinase-3.Oncogene · 2004Article
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6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At the onset of mitosis, the Golgi apparatus, which consists of several cisternae, disperses throughout the cell to be partitioned into daughter cells. The molecular mechanisms of this process are now beginning to be understood. To investigate the biochemical requirements and kinetics of mitotic Golgi membrane dynamics in polarized cells, we have reconstituted the disassembly of the Golgi apparatus by introducing Xenopus egg extracts into permeabilized Mardin-Darby canine kidney (MDCK) cells. We used green fluorescence protein (GFP)-tagged galactosyltransferase-expressing MDCK cells to analyze the morphological changes of the Golgi membrane in the semi-intact system. Analyses by fluorescence and electron microscopies showed that the Golgi disassembly can be dissected into two elementary processes morphologically. In the first process, the perinuclear Golgi stacks break into punctate structures, intermediates, which are comprised of mini-stacks of cisternae associating with apical microtubule networks. In the second process, the structures fragment more thoroughly or substantially relocate to the ER. Our analyses further showed that cdc2 kinase and mitogen-activated protein kinase kinase (MAPKK = MEK) are differently involved in these two processes: the first process is mainly regulated by MEK and the second mainly by cdc2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.