ArticleInfection and immunity2000
Characterization of heat, oxidative, and acid stress responses in Brucella melitensis.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 101 citations in OpenAlex.
- A Putative Multicopper Oxidase Encoded byInternational journal of molecular sciences · 2026Article
- Lacticaseibacillus paracasei AD22 Stress Response in Brined White Cheese Matrix: In Vitro Probiotic Profiles and Molecular Characterization.Probiotics and antimicrobial proteins · 2025Article
- Analysis of stress response in multiple bacterial pathogens using a network biology approach.Scientific reports · 2025Article
- Superoxide dismutase VPA1514 in Vibrio parahaemolyticus protects against environmental stresses.PloS one · 2025Article
- Immuno-profiling ofFrontiers in microbiology · 2023Article
- Brucella melitensis invA gene (BME_RS01060) transcription is promoted under acidic stress conditions.Archives of microbiology · 2021Article
- Comparative Genome-Wide Transcriptome Analysis ofFrontiers in microbiology · 2021Article
- Deletion of the Transcriptional Regulator MucR inFrontiers in veterinary science · 2021Article
- Genomic Analysis of Natural RoughInternational journal of molecular sciences · 2020Article
- The Twin-Arginine Translocation System Is Important for Stress Resistance and Virulence of Brucella melitensis.Infection and immunity · 2020Article
- Pan-Proteomic Analysis and Elucidation of Protein Abundance among the Closely RelatedBiomolecules · 2020Article
- Comparative transcriptomic and proteomic analyses reveal upregulated expression of virulence and iron transport factors of Aeromonas hydrophila under iron limitation.BMC microbiology · 2018Article
- Dissecting the Acid Stress Response ofFrontiers in microbiology · 2018Article
- Relative Quantitative Proteomic Analysis ofFrontiers in microbiology · 2017Article
- A Novel Small RNA Regulates Tolerance and Virulence in Shigella flexneri by Responding to Acidic Environmental Changes.Frontiers in cellular and infection microbiology · 2016Article
- RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress.Scientific reports · 2015Article
- Multiple chaperonins in bacteria--novel functions and non-canonical behaviors.Cell stress & chaperones · 2015Review
- Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle.Veterinary research · 2015Article
- Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis.Frontiers in microbiology · 2015Article
- The invA gene of Brucella melitensis is involved in intracellular invasion and is required to establish infection in a mouse model.Virulence · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. Therefore, it has to adapt to a range of different hostile environments. In order to understand the mechanisms of intracellular survival employed by virulent B. melitensis 16M, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid pH stresses by two-dimensional (2-D) polyacrylamide gel electrophoresis was used. Depending on the stress, this involved about 6.4 to 12% of the 676 protein spots detected in 2-D gel electrophoresis. On the basis of N-terminal sequence analysis and database searching, 19 proteins whose level of synthesis was up- or down-regulated by stress conditions were identified. Some of them were previously reported for Brucella, such as BvrR, DnaK, GroEL, and Cu-Zn superoxide dismutase (SOD). Eight other proteins closely matched proteins found in other bacteria: AapJ, alpha-ETF, ClpP, Fe and/or Mn SOD, malate dehydrogenase, IalB, 30S ribosomal protein S1, and pyruvate dehydrogenase E1 component beta subunit. Results indicated that B. melitensis could bring specific regulatory mechanisms into play in response to stress conditions. For example, the ribosome releasing factor in B. melitensis appeared to be a heat shock protein, whereas the ClpP protein, described as a heat shock protein for Escherichia coli, was strongly down-regulated in B. melitensis in response to heat stress. Some of the identified proteins and their potential specific regulation could be required for the adaptation of B. melitensis to environmental stresses encountered in phagocytic cells and possibly for bacterial virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.