ArticleInfection and immunity2000
Serological expression cloning of novel immunoreactive antigens of Babesia microti.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Major Surface Antigens in ZoonoticPathogens (Basel, Switzerland) · 2022Review
- Technologies for Detection ofPathogens (Basel, Switzerland) · 2021Review
- Review
- Evidence for vesicle-mediated antigen export by the human pathogenLife science alliance · 2019Article
- Comparative 3D genome organization in apicomplexan parasites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A library of recombinant Babesia microti cell surface and secreted proteins for diagnostics discovery and reverse vaccinology.International journal for parasitology · 2019Article
- Surface Antigen 1 Is a Crucial Secreted Protein That MediatesFrontiers in microbiology · 2019Article
- BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection.Journal of clinical microbiology · 2018Article
- Performance Evaluation of a Prototype Architect Antibody Assay for Babesia microti.Journal of clinical microbiology · 2018Article
- A multiplex serologic platform for diagnosis of tick-borne diseases.Scientific reports · 2018Article
- Evaluation of a Major Surface Antigen ofFrontiers in microbiology · 2017Article
- Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions.Scientific reports · 2016Article
- A targeted immunomic approach identifies diagnostic antigens in the human pathogen Babesia microti.Transfusion · 2016Article
- Serologic screening of United States blood donors for Babesia microti using an investigational enzyme immunoassay.Transfusion · 2016Article
- Determination of Babesia microti seroprevalence in blood donor populations using an investigational enzyme immunoassay.Transfusion · 2014Article
- Whole genome mapping and re-organization of the nuclear and mitochondrial genomes of Babesia microti isolates.PloS one · 2013Article
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Authors and funding
9 authors.
Funding
Abstract
Increased recognition of the prevalence of human babesiosis in the United States, together with rising concern about the potential for transmission of this infection by blood transfusion, has provided motivation to develop definitive serologic and molecular tests for the causative agent, Babesia microti. To develop more sensitive and specific assays for B. microti, we screened a genomic expression library with patient serum pools. This screening resulted in the identification of three classes of novel genes and an additional two novel, unrelated genes, which together encode a total of 17 unique B. microti antigens. The first class (BMN1-2 family) of genes encodes seven closely related antigens with a degenerate six-amino-acid repeat that shows limited homology to Plasmodium sp. merozoite and sporozoite surface antigens. A second class (BMN1-8 family) of genes encodes six related antigens, and the third class (BMN1-17 family) of genes encodes two related antigens. The two remaining genes code for novel and unrelated sequences. Among the three classes of antigens and remaining novel sequences, five were chosen to code for the most immunodominant antigens (BMN1-2, -9, -15, and -17 and MN-10). Western blot analysis with the resulting recombinant proteins indicated that these antigens were targets of humoral immune responses during B. microti infection in humans.
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