ArticleInfection and immunity2000
Heterologous expression of Trypanosoma cruzi trans-sialidase in Leishmania major enhances virulence.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Use ofFrontiers in microbiology · 2022Article
- Major Molecular Factors Related toFrontiers in immunology · 2022Review
- Leishmania tarentolae: Taxonomic classification and its application as a promising biotechnological expression host.PLoS neglected tropical diseases · 2019Review
- Costimulatory Effects of an Immunodominant Parasite Antigen Paradoxically Prevent Induction of Optimal CD8 T Cell Protective Immunity.PLoS pathogens · 2016Article
- Free energy study of the catalytic mechanism of Trypanosoma cruzi trans-sialidase. From the Michaelis complex to the covalent intermediate.Biochemistry · 2011Article
- Trypanosoma cruzi-Derived Neurotrophic Factor: Role in Neural Repair and Neuroprotection.Journal of neuroparasitology · 2010Article
- Upregulated expression of B-cell antigen family tandem repeat proteins by Leishmania amastigotes.Infection and immunity · 2010Article
- Recombinant protein expression in Leishmania tarentolae.Molecular biotechnology · 2009Review
- The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes.Nucleic acids research · 2008Article
- Virulence attenuation of a UDP-galactose/N-acetylglucosamine beta1,4 galactosyltransferase expressing Leishmania donovani promastigote.Glycoconjugate journal · 2008Article
- A Leishmania infantum cytosolic tryparedoxin activates B cells to secrete interleukin-10 and specific immunoglobulin.Immunology · 2008Article
- Transgenic Leishmania and the immune response to infection.Parasite immunology · 2008Review
- CD8+ T-Cell responses to Trypanosoma cruzi are highly focused on strain-variant trans-sialidase epitopes.PLoS pathogens · 2006Article
- Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages.Parasitology research · 2005Article
- Sialoglycans in protozoal diseases: their detection, modes of acquisition and emerging biological roles.Glycoconjugate journal · 2004Review
- Thymocyte depletion in Trypanosoma cruzi infection is mediated by trans-sialidase-induced apoptosis on nurse cells complex.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Trypanosoma cruzi trans-sialidase: a potent and specific survival factor for human Schwann cells by means of phosphatidylinositol 3-kinase/Akt signaling.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Epitope mapping of trans-sialidase from Trypanosoma cruzi reveals the presence of several cross-reactive determinants.Infection and immunity · 2001Article
- Achievements and challenges of sialic acid research.Glycoconjugate journalReview
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Earlier studies showed that mice primed for a few hours with the trans-sialidase (TS) of Trypanosoma cruzi, the agent of Chagas' disease, become highly susceptible to trypanosomal infection. These studies suggest that TS affects parasite virulence independent of antigenic stimulation. Potentially, TS could enhance or reduce the virulence of heterologous microbes depending on the mechanism of TS action and on the type of immune response elicited by the particular parasite. We tested this hypothesis by expressing heterologous TS in Leishmania major, a protozoan parasite that causes cutaneous leishmaniasis and lacks TS and the TS product alpha2-3-linked sialic acid. Leishmania cells transfected with a T. cruzi TS expression construct made high levels of active enzyme, which was present in the promastigotes and shed into the extracellular milieu. TS expression did not affect L. major binding to and entry into cultured macrophages or its tropism for macrophage infection in vivo. However, TS-expressing L. major exhibited elevated virulence in BALB/c mice, as determined by lesion progression, parasite numbers, and macro- and microscopic examination of cutaneous lesions. Several genetic tests proved that the enhanced virulence was directly attributable to TS expression. The results are consistent with TS functioning to sabotage the mouse immune system to confer a growth advantage on T. cruzi and transgenic L. major. These data suggest that heterologous expression of T. cruzi virulence factors in Leishmania may provide a new approach for dissecting their function in vivo.
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