ArticleInfection and immunity2000
Nitric oxide participation in the fungicidal mechanism of gamma interferon-activated murine macrophages against Paracoccidioides brasiliensis conidia.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 149 citations in OpenAlex.
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- Invariant Natural Killer T Cells as Key Players in Host Resistance againstJournal of immunology research · 2021Article
- Chronic mucocutaneous candidiasis associated with paracoccidioidomycosis in a patient with mannose receptor deficiency: First case reported in the literature.Revista da Sociedade Brasileira de Medicina Tropical · 2021Article
- iNOS/Arginase-1 expression in the pulmonary tissue over time duringInnate immunity · 2020Article
- The Trojan Horse Model inFrontiers in cellular and infection microbiology · 2020Review
- Overview of β-Glucans from Laminaria spp.: Immunomodulation Properties and Applications on Biologic Models.International journal of molecular sciences · 2017Review
- The IDO-AhR Axis Controls Th17/Treg Immunity in a Pulmonary Model of Fungal Infection.Frontiers in immunology · 2017Article
- Th17-Inducing Cytokines IL-6 and IL-23 Are Crucial for Granuloma Formation during Experimental Paracoccidioidomycosis.Frontiers in immunology · 2017Article
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- Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected with Paracoccidioides brasiliensis.Mediators of inflammation · 2016Article
- Anti-Immune Strategies of Pathogenic Fungi.Frontiers in cellular and infection microbiology · 2016Review
- THE POWER OF THE SMALL: THE EXAMPLE OF Paracoccidioides brasiliensis CONIDIA.Revista do Instituto de Medicina Tropical de Sao Paulo · 2015Review
- Involvement of major components from Sporothrix schenckii cell wall in the caspase-1 activation, nitric oxide and cytokines production during experimental sporotrichosis.Mycopathologia · 2015Article
- Saccharomyces cerevisiae expressing Gp43 protects mice against Paracoccidioides brasiliensis infection.PloS one · 2015Article
- rPbPga1 from Paracoccidioides brasiliensis Activates Mast Cells and Macrophages via NFkB.PLoS neglected tropical diseases · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paracoccidioidomycosis, a systemic mycosis restricted to Latin America and produced by the dimorphic fungus Paracoccidioides brasiliensis, is probably acquired by inhalation of conidia produced by the mycelial form. The macrophage (Mphi) represents the major cell defense against this pathogen; when activated with gamma interferon (IFN-gamma), murine Mphis kill the fungus by an oxygen-independent mechanism. Our goal was to determine the role of nitric oxide in the fungicidal effect of Mphis on P. brasiliensis conidia. The results revealed that IFN-gamma-activated murine Mphis inhibited the conidium-to-yeast transformation process in a dose-dependent manner; maximal inhibition was observed in Mphis activated with 50 U/ml and incubated for 96 h at 37 degrees C. When Mphis were activated with 150 to 200 U of cytokine per ml, the number of CFU was 70% lower than in nonactivated controls, indicating that there was a fungicidal effect. The inhibitory effect was reversed by the addition of anti-IFN-gamma monoclonal antibodies. Activation by IFN-gamma also enhanced Mphi nitric oxide production, as revealed by increasing NO(2) values (8 +/- 3 microM in nonactivated Mphis versus 43 +/- 13 microM in activated Mphis). The neutralization of IFN-gamma also reversed nitric oxide production at basal levels (8 +/- 5 microM). Additionally, we found that there was a significant inverse correlation (r = -0.8975) between NO(2)(-) concentration and transformation of P. brasiliensis conidia. Additionally, treatment with any of the three different nitric oxide inhibitors used (arginase, N(G)-monomethyl-L-arginine, and aminoguanidine), reverted the inhibition of the transformation process with 40 to 70% of intracellular yeast and significantly reduced nitric oxide production. These results show that IFN-gamma-activated murine Mphis kill P. brasiliensis conidia through the L-arginine-nitric oxide pathway.
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