ArticleTransgenic research1999
Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies.
Article in Transgenic research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 136 citations in OpenAlex.
- The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.Frontiers in bioengineering and biotechnology · 2026Review
- Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems.Heliyon · 2024Review
- The advent of plant cells in bioreactors.Frontiers in plant science · 2023Review
- Compendium on Food Crop Plants as a Platform for Pharmaceutical Protein Production.International journal of molecular sciences · 2022Review
- Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production.Plants (Basel, Switzerland) · 2020Review
- Neosaxitoxin Inhibits the Expression of Inflammation Markers of the M1 Phenotype in Macrophages.Marine drugs · 2020Article
- Reconceptualizing cancer immunotherapy based on plant production systems.Future science OA · 2017Review
- Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.Plant biotechnology journal · 2016Article
- Production of monoclonal antibodies in plants for cancer immunotherapy.BioMed research international · 2015Review
- Transgenic rice endosperm as a bioreactor for molecular pharming.Plant cell reports · 2014Review
- Expression of recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi.Molecular biology reports · 2013Article
- Improving pharmaceutical protein production in Oryza sativa.International journal of molecular sciences · 2013Review
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- Functional characterization of the recombinant HIV-neutralizing monoclonal antibody 2F5 produced in maize seeds.Plant molecular biology · 2012Article
- Development of an optimized tetracycline-inducible expression system to increase the accumulation of interleukin-10 in tobacco BY-2 suspension cells.BMC biotechnology · 2012Article
- Tobacco as biofactory for biologically active hPL production: a human hormone with potential applications in type-1 diabetes.Transgenic research · 2011Article
- Expression of anti-K99 scFv in transgenic rice tissues and its functional characterization.Transgenic research · 2009Article
- Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture.Plant molecular biology · 2008Article
- Bioproduction of therapeutic proteins in the 21st century and the role of plants and plant cells as production platforms.Annals of the New York Academy of Sciences · 2007Review
- Plant-derived anti-Lewis Y mAb exhibits biological activities for efficient immunotherapy against human cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the suitability of transformed rice cell lines as a system for the production of therapeutic recombinant antibodies. Expression constructs encoding a single-chain Fv fragment (scFvT84.66, specific for CEA, the carcinoembryonic antigen present on many human tumours) were introduced into rice tissue by particle bombardment. We compared antibody production levels when antibodies were either secreted to the apoplast or retained in the endoplasmic reticulum (ER) using a KDEL retention signal. Production levels were up to 14 times higher when antibodies were retained in the ER. Additionally, we compared construct sencoding different leader peptides (plant codon optimised murine immunoglobulin heavy and light chain leader peptides from mAb24) and carrying alternative 5' untranslated regions (the petunia chalcone synthase gene 5' UTR and the tobacco mosaic virus omega sequence). We observed no significant differences in antibody production levels among cell lines transformed with these constructs. The highest level of antibody production we measured was 3.8 micrograms g-1 callus (fresh weight). Immunological analysis of transgenic rice callus confirmed the presence of functional scFvT84.66. We discuss the potential merits of cell culture for the production of recombinant antibodies and other valuable macromolecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.