ArticleThe Journal of physiology2000
Corticosteroids decrease mRNA levels of SERCA pumps, whereas they increase sarcolipin mRNA in the rat diaphragm.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Curcumin Administration Improves Force ofAntioxidants (Basel, Switzerland) · 2023Article
- Prenatal metyrapone treatment modulates neonatal cerebrovascular structure, function, and vulnerability to mild hypoxic-ischemic injury.American journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Sarcolipin deletion in mdx mice impairs calcineurin signalling and worsens dystrophic pathology.Human molecular genetics · 2018Article
- Sarcolipin expression is repressed by endoplasmic reticulum stress in C2C12 myotubes.Journal of physiology and biochemistry · 2017Article
- Reducing sarcolipin expression mitigates Duchenne muscular dystrophy and associated cardiomyopathy in mice.Nature communications · 2017Article
- Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice.PloS one · 2017Article
- Sarcolipin: A Key Thermogenic and Metabolic Regulator in Skeletal Muscle.Trends in endocrinology and metabolism: TEM · 2016Review
- Effects of cyclopiazonic acid and dexamethasone on serotonin-induced calcium responses in vascular smooth muscle cells.Journal of physiology and biochemistry · 2016Article
- Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.The Journal of biological chemistry · 2015Article
- The neonatal sarcoplasmic reticulum Ca2+-ATPase gives a clue to development and pathology in human muscles.Journal of muscle research and cell motility · 2015Article
- Structural and dynamic basis of phospholamban and sarcolipin inhibition of Ca(2+)-ATPase.Biochemistry · 2008Review
- An electrochemical investigation of sarcolipin reconstituted into a mercury-supported lipid bilayer.Biophysical journal · 2007Article
- Differential expression of sarcolipin protein during muscle development and cardiac pathophysiology.Journal of molecular and cellular cardiology · 2007Article
- Sarcolipin and phospholamban as regulators of cardiac sarcoplasmic reticulum Ca2+ ATPase.Journal of molecular and cellular cardiology · 2007Review
- Sarcolipin and phospholamban mRNA and protein expression in cardiac and skeletal muscle of different species.The Biochemical journal · 2005Article
- Cardiac-specific overexpression of sarcolipin inhibits sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2a) activity and impairs cardiac function in mice.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Sarcolipin regulates sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) by binding to transmembrane helices alone or in association with phospholamban.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Sarcolipin uncouples hydrolysis of ATP from accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum.The Biochemical journal · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. In order to explore the potential role of the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA)-type pumps and of their modulators phospholamban (PLB) and sarcolipin (SLN) in the functional alterations of the diaphragm induced by corticosteroid treatment, expression of SERCA, PLB and SLN was assessed by RT-PCR in the diaphragm of rats treated daily for 5 days either with triamcinolone (80 mg kg-1, n = 8) or with saline (control; 0.6 ml, n = 8). 2. Triamcinolone treatment reduced the normalised overall amount of all SERCA mRNA in diaphragm by 70 % compared to controls (P < 0.05). This reduction was accounted for by a relatively larger decrease in the SERCA1 mRNA (-69 %, P < 0.05) whilst the decrease in SERCA2 mRNA (-49 %, P = 0.09) did not reach statistical significance. As a result the relative proportion of SERCA2 mRNA was increased from 43 +/- 7 % in control diaphragm to 52 +/- 4 % after triamcinolone treatment (P < 0.05). 3. Only the adult isoform of SERCA1 (i.e. SERCA1a) mRNA was found in the diaphragm of the 15-week-old control rats. Furthermore, triamcinolone treatment resulted in reduced levels of SERCA2a (-40 %, P < 0.05) and increased levels of SLN mRNA (+100 %, P < 0.05), while the decrease in PLB mRNA (-31 %, P = 0.277) did not reach statistical significance. SERCA1b, SERCA2b and SERCA3 mRNA levels fell below the detection limit in the diaphragm of both control and triamcinolone-treated rats. 4. Compared to control diaphragm, control rat heart showed a relatively high PLB/(SERCA1 + SERCA2) mRNA ratio of 7.88 while this ratio amounted only to 0.16 in control extensor digitorum longus (EDL) muscle. Remarkably, the SLN/(SERCA1 + SERCA2) mRNA ratio in normal cardiac muscle (0.96) was nearly the same as in diaphragm, but in EDL it amounted to only 0.05 that in diaphragm. This indicates the very low expression of SLN in rat EDL. 5. These data reveal that considerable alterations in SERCA mRNA levels accompany the functional changes seen in diaphragm after corticosteroid treatment. The relatively larger decrease in SERCA1 mRNA is in agreement with the selective type II fibre atrophy previously observed in the diaphragm of triamcinolone-treated rats, but the magnitude of SERCA alterations is more pronounced than expected on the basis of the structural changes in the diaphragm. The increase in SLN mRNA levels may represent a compensatory mechanism.
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