ArticleGenes & development2000
The Drosophila HEM-2/NAP1 homolog KETTE controls axonal pathfinding and cytoskeletal organization.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 82 citations in OpenAlex.
- Hem1 inborn errors of immunity: waving goodbye to coordinated immunity in mice and humans.Frontiers in immunology · 2024Review
- Role of NCKAP1 in the Defective Phagocytic Function of Microglia-Like Cells Derived from Rapidly Progressing Sporadic ALS.Molecular neurobiology · 2023Article
- Orchestration of synaptic functions by WAVE regulatory complex-mediated actin reorganization.Experimental & molecular medicine · 2023Review
- Tuning the rheostat of immune gene translation.Stress biology · 2023Article
- Vav independently regulates synaptic growth and plasticity through distinct actin-based processes.The Journal of cell biology · 2022Article
- NCKAP1 Disruptive Variants Lead to a Neurodevelopmental Disorder with Core Features of Autism.American journal of human genetics · 2020Article
- The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.Cells · 2020Article
- The Formin Diaphanous Regulates Myoblast Fusion through Actin Polymerization and Arp2/3 Regulation.PLoS genetics · 2015Article
- Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion.BMC cell biology · 2014Article
- PI(4,5)P2 regulates myoblast fusion through Arp2/3 regulator localization at the fusion site.Development (Cambridge, England) · 2014Article
- Live imaging provides new insights on dynamic F-actin filopodia and differential endocytosis during myoblast fusion in Drosophila.PloS one · 2014Article
- SCAR/WAVE-mediated processing of engulfed apoptotic corpses is essential for effective macrophage migration in Drosophila.Cell death and differentiation · 2013Article
- Group I PAKs function downstream of Rac to promote podosome invasion during myoblast fusion in vivo.The Journal of cell biology · 2012Article
- Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila.Molecular biology of the cell · 2011Article
- The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase.Developmental biology · 2011Article
- The actin nucleator WASp is required for myoblast fusion during adult Drosophila myogenesis.Development (Cambridge, England) · 2011Article
- Competition between Blown fuse and WASP for WIP binding regulates the dynamics of WASP-dependent actin polymerization in vivo.Developmental cell · 2011Article
- The glucuronyltransferase GlcAT-P is required for stretch growth of peripheral nerves in Drosophila.PloS one · 2011Article
- Review
- An invasive podosome-like structure promotes fusion pore formation during myoblast fusion.The Journal of cell biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Drosophila, the correct formation of the segmental commissures depends on neuron-glial interactions at the midline. The VUM midline neurons extend axons along which glial cells migrate in between anterior and posterior commissures. Here, we show that the gene kette is required for the normal projection of the VUM axons and subsequently disrupts glial migration. Axonal projection defects are also found for many other moto- and interneurons. In addition, kette affects the cell morphology of mesodermal and epidermal derivatives, which show an abnormal actin cytoskeleton. The KETTE protein is homologous to the transmembrane protein HEM-2/NAP1 evolutionary conserved from worms to vertebrates. In vitro analysis has shown a specific interaction of the vertebrate HEM-2/NAP1 with the SH2-SH3 adapter protein NCK and the small GTPase RAC1, which both have been implicated in regulating cytoskeleton organization and axonal growth. Hypomorphic kette mutations lead to axonal defects similar to mutations in the Drosophila NCK homolog dreadlocks. Furthermore, we show that kette and dock mutants genetically interact. NCK is thought to interact with the small G proteins RAC1 and CDC42, which play a role in axonal growth. In line with these observations, a kette phenocopy can be obtained following directed expression of mutant DCDC42 or DRAC1 in the CNS midline. In addition, the kette mutant phenotype can be partially rescued by expression of an activated DRAC1 transgene. Our data suggest an important role of the HEM-2 protein in cytoskeletal organization during axonal pathfinding.
Indexed as
Identifiers
10766742PMC316499W2149119048What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.