ArticleGenes & development2000
Ribosome shunting in the cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 88 citations in OpenAlex.
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- TOR senses and regulates spermidine metabolism during seedling establishment and growth in maize and Arabidopsis.iScience · 2021Article
- Cell-free riboswitches.RSC chemical biology · 2021Review
- Control of translation by eukaryotic mRNA transcript leaders-Insights from high-throughput assays and computational modeling.Wiley interdisciplinary reviews. RNA · 2021Review
- Noncanonical Translation Initiation in Eukaryotes.Cold Spring Harbor perspectives in biology · 2019Review
- Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g.Viruses · 2018Article
- Translation acrobatics: how cancer cells exploit alternate modes of translational initiation.EMBO reports · 2018Review
- Conserved non-AUG uORFs revealed by a novel regression analysis of ribosome profiling data.Genome research · 2018Article
- Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond.Frontiers in microbiology · 2018Review
- Badnaviruses: The Current Global Scenario.Viruses · 2016Review
- TOR and S6K1 promote translation reinitiation of uORF-containing mRNAs via phosphorylation of eIF3h.The EMBO journal · 2013Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
- Short ORF-dependent ribosome shunting operates in an RNA picorna-like virus and a DNA pararetrovirus that cause rice tungro disease.PLoS pathogens · 2012Article
- Viral subversion of the host protein synthesis machinery.Nature reviews. Microbiology · 2011Review
- RNA sequence determinants of a coupled termination-reinitiation strategy for downstream open reading frame translation in Helminthosporium victoriae virus 190S and other victoriviruses (Family Totiviridae).Journal of virology · 2011Article
- Identification of potential conserved RNA secondary structure throughout influenza A coding regions.RNA (New York, N.Y.) · 2011Article
- Molecular dissection of the prototype foamy virus (PFV) RNA 5'-UTR identifies essential elements of a ribosomal shunt.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The shunt model predicts that small ORFs (sORFs) within the cauliflower mosaic virus (CaMV) 35S RNA leader and downstream ORF VII are translated by different mechanisms, that is, scanning-reinitiation and shunting, respectively. Wheat germ extract (WGE) and rabbit reticulocyte lysate (RRL) in vitro translation systems were used to discriminate between these two processes and to study the mechanism of ribosomal shunt. In both systems, expression downstream of the leader occurred via ribosomal shunt under the control of a stable stem and a small ORF preceding it. Shunting ribosomes were also able to initiate quite efficiently at non-AUG start codons just downstream of the shunt landing site in WGE but not in RRL. The short sORF MAGDIS from the mammalian AdoMetDC RNA, which conditionally suppresses reinitiation at a downstream ORF, prevented shunting if placed at the position of sORF A, the 5'-proximal ORF of the CaMV leader. We have demonstrated directly that sORF A is translated and that proper termination of translation at the 5'-proximal ORF is absolutely required for both shunting and linear ribosome migration. These findings strongly indicate that shunting is a special case of reinitiation.
Indexed as
Identifiers
10766738PMC316492W2119698111What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.