ArticleJournal of bacteriology2000
Calcofluor antifungal action depends on chitin and a functional high-osmolarity glycerol response (HOG) pathway: evidence for a physiological role of the Saccharomyces cerevisiae HOG pathway under noninducing conditions.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Insight into the role of Sho1 in high-hydrostatic pressure and oxidative stress adaptation of the hadal fungusApplied and environmental microbiology · 2026Article
- Osmotic Stress Responses, Cell Wall Integrity, and Conidiation Are Regulated by a Histidine Kinase Sensor inJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Inhibition of Dephosphorylation of Dolichyl Diphosphate Alters the Synthesis of Dolichol and Hinders ProteinInternational journal of molecular sciences · 2019Article
- The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi.Frontiers in cellular and infection microbiology · 2019Review
- Influence of ylHog1 MAPK kinase on Yarrowia lipolytica stress response and erythritol production.Scientific reports · 2018Article
- Impact of Fungal MAPK Pathway Targets on the Cell Wall.Journal of fungi (Basel, Switzerland) · 2018Article
- Resistance to the Plant Defensin NaD1 Features Modifications to the Cell Wall and Osmo-Regulation Pathways of Yeast.Frontiers in microbiology · 2018Article
- Cinnamic Acid Analogs as Intervention Catalysts for Overcoming Antifungal Tolerance.Molecules (Basel, Switzerland) · 2017Article
- The Mitogen-Activated Protein Kinase Kinase VdPbs2 ofFrontiers in microbiology · 2016Article
- Augmenting the Activity of Monoterpenoid Phenols against Fungal Pathogens Using 2-Hydroxy-4-methoxybenzaldehyde that Target Cell Wall Integrity.International journal of molecular sciences · 2015Article
- Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae.BMC microbiology · 2014Article
- Role of LAMMER Kinase in Cell Wall Biogenesis during Vegetative Growth of Aspergillus nidulans.Mycobiology · 2014Article
- Using Aspergillus nidulans to identify antifungal drug resistance mutations.Eukaryotic cell · 2014Article
- Calcofluor white combination antifungal treatments for Trichophyton rubrum and Candida albicans.PloS one · 2012Article
- Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signaling pathway.Genetics · 2011Review
- Melanin externalization in Candida albicans depends on cell wall chitin structures.Eukaryotic cell · 2010Article
- The activity of yeast Hog1 MAPK is required during endoplasmic reticulum stress induced by tunicamycin exposure.The Journal of biological chemistry · 2010Article
- Fungal echinocandin resistance.Fungal genetics and biology : FG & B · 2010Article
- Slt2 and Rim101 contribute independently to the correct assembly of the chitin ring at the budding yeast neck in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
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3 authors.
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Abstract
We have isolated several Saccharomyces cerevisiae mutants resistant to calcofluor that contain mutations in the PBS2 or HOG1 genes, which encode the mitogen-activated protein kinase (MAPK) and MAP kinases, respectively, of the high-osmolarity glycerol response (HOG) pathway. We report that blockage of either of the two activation branches of the pathway, namely, SHO1 and SLN1, leads to partial resistance to calcofluor, while simultaneous disruption significantly increases resistance. However, chitin biosynthesis is independent of the HOG pathway. Calcofluor treatment also induces an increase in salt tolerance and glycerol accumulation, although no activation of the HOG pathway is detected. Our results indicate that the antifungal effect of calcofluor depends on its binding to cell wall chitin but also on the presence of a functional HOG pathway. Characterization of one of the mutants isolated, pbs2-14, revealed that resistance to calcofluor and HOG-dependent osmoadaptation are two different physiological processes. Sensitivity to calcofluor depends on the constitutive functionality of the HOG pathway; when this is altered, the cells become calcofluor resistant but also show very low levels of basal salt tolerance. Characterization of some multicopy suppressors of the calcofluor resistance phenotype indicated that constitutive HOG functionality participates in the maintenance of cell wall architecture, a conclusion supported by the antagonism observed between the protein kinase and HOG signal transduction pathways.
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