ArticleProceedings of the National Academy of Sciences of the United States of America2000
Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.
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157 citing papers in PubMed, 429 citations in OpenAlex.
- Trans-2-hexenal priming supports HSFA2-independent thermotolerance in Arabidopsis thaliana.Journal of plant research · 2026Article
- Gene Families, Structural and Functional Contexts Shape Protein Thermostability and Paralog Divergence inPlants (Basel, Switzerland) · 2026Article
- FaCPK19-FaTT1 axis decodes heat-induced CaThe New phytologist · 2026Article
- Selection of heat stress-tolerant cultivars from radish and analysis of gene expression under high-temperature.Genes & genomics · 2026Article
- Natural variation uncouples HSFA2 induction from volatile-induced thermotolerance in Arabidopsis thaliana.Plant molecular biology · 2026Article
- From Perception to Adaptation: A Comparative Study of Plant Regulatory Networks in Response to Heat and Waterlogging Stress.Plants (Basel, Switzerland) · 2026Review
- Transcriptomic analysis of reduced thermotolerance inFrontiers in plant science · 2026Article
- Changes in both gene expression and intron retention tune themicroPublication biology · 2026Article
- Integrating multiomic resources and gene expression studies to identify candidates for engineering climate-resilientFrontiers in plant science · 2026Review
- Analysis of plant gene family heat shock protein 100 (HSP100) and its orthologs in Eukarya reveals sites of divergent evolution and insights into endosymbiotic origins of chloroplasts.Plant signaling & behavior · 2025Article
- Chloroplast precursor protein preClpD overaccumulation triggers multilevel reprogramming of gene expression and a heat shock-like response.Nature communications · 2025Article
- AtMYBS1 negatively regulates heat tolerance by directly repressing the expression of MAX1 required for strigolactone biosynthesis in Arabidopsis.Plant communications · 2023Article
- A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance.Plant methods · 2023Article
- CLPB3 is required for the removal of chloroplast protein aggregates and thermotolerance in Chlamydomonas.Journal of experimental botany · 2023Article
- Genomic and epigenomic determinants of heat stress-induced transcriptional memory in Arabidopsis.Genome biology · 2023Article
- Article
- Identification of subfunctionalized aggregate-remodeling J-domain proteins in Arabidopsis thaliana.Journal of experimental botany · 2023Article
- Facilitated Adaptation as A Conservation Tool in the Present Climate Change Context: A Methodological Guide.Plants (Basel, Switzerland) · 2023Review
- Mutation of the polyadenylation complex subunit CstF77 reveals that mRNA 3' end formation and HSP101 levels are critical for a robust heat stress response.The Plant cell · 2023Article
- Maintenance of abiotic stress memory in plants: Lessons learned from heat acclimation.The Plant cell · 2023Review
97 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of organisms to acquire thermotolerance to normally lethal high temperatures is an ancient and conserved adaptive response. However, knowledge of cellular factors essential to this response is limited. Acquisition of thermotolerance is likely to be of particular importance to plants that experience daily temperature fluctuations and are unable to escape to more favorable environments. We developed a screen, based on hypocotyl elongation, for mutants of Arabidopsis thaliana that are unable to acquire thermotolerance to high-temperature stress and have defined four separate genetic loci, hot1-4, required for this process. hot1 was found to have a mutation in the heat shock protein 101 (Hsp101) gene, converting a conserved Glu residue in the second ATP-binding domain to a Lys residue, a mutation that is predicted to compromise Hsp101 ATPase activity. In addition to exhibiting a thermotolerance defect as assayed by hypocotyl elongation, 10-day-old hot1 seedlings were also unable to acquire thermotolerance, and hot1 seeds had greatly reduced basal thermotolerance. Complementation of hot1 plants by transformation with wild-type Hsp101 genomic DNA restored hot1 plants to the wild-type phenotype. The hot mutants are the first mutants defective in thermotolerance that have been isolated in a higher eukaryote, and hot1 represents the first mutation in an Hsp in any higher plant. The phenotype of hot1 also provides direct evidence that Hsp101, which is required for thermotolerance in bacteria and yeast, is also essential for thermotolerance in a complex eukaryote.
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