ArticleProceedings of the National Academy of Sciences of the United States of America2000
Heteroduplex mobility assay-guided sequence discovery: elucidation of the small subunit (18S) rDNA sequences of Pfiesteria piscicida and related dinoflagellates from complex algal culture and environmental sample DNA pools.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals.Environmental microbiology · 2012Article
- Temporal distribution of genetically homogenous 'free-living' Hematodinium sp. in a Delmarva coastal ecosystem.Aquatic biosystems · 2012Article
- Molecular detection, quantification, and diversity evaluation of microalgae.Marine biotechnology (New York, N.Y.) · 2012Review
- Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology.Limnology and oceanography, methods · 2010Article
- Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis.Harmful algae · 2009Article
- Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida.Applied and environmental microbiology · 2007Article
- RAPHIDOPHYCEAE [CHADEFAUD EX SILVA] SYSTEMATICS AND RAPID IDENTIFICATION: SEQUENCE ANALYSES AND REAL-TIME PCR ASSAYS.Journal of phycology · 2006Article
- Occupational exposure to pfiesteria species in estuarine waters is not a risk factor for illness.Environmental health perspectives · 2006Article
- Development of a cob-18S rRNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment.Applied and environmental microbiology · 2005Article
- Demonstration of toxicity to fish and to mammalian cells by Pfiesteria species: comparison of assay methods and strains.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Modified serial analysis of gene expression method for construction of gene expression profiles of microbial eukaryotic species.Applied and environmental microbiology · 2004Article
- Comparative impacts of two major hurricane seasons on the Neuse River and western Pamlico Sound ecosystems.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the nontranscribed spacer.Applied and environmental microbiology · 2002Article
- Detection and quantification of Pfiesteria piscicida by using the mitochondrial cytochrome b gene.Applied and environmental microbiology · 2002Article
- Discovery of the toxic dinoflagellate Pfiesteria in northern European waters.Proceedings. Biological sciences · 2002Article
- Pfiesteria: review of the science and identification of research gaps. Report for the National Center for Environmental Health, Centers for Disease Control and Prevention.Environmental health perspectives · 2001Review
- Environmental public health surveillance: possible estuary-associated syndrome.Environmental health perspectives · 2001Article
- Cohort studies of health effects among people exposed to estuarine waters: North Carolina, Virginia, and Maryland.Environmental health perspectives · 2001Article
- Fish bioassay and toxin induction experiments for research on Pfiesteria piscicida and other toxic dinoflagellates: workshop summary.Environmental health perspectives · 2001Article
- Use of molecular probes to assess geographic distribution of Pfiesteria species.Environmental health perspectives · 2001Article
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Authors and funding
12 authors.
Funding
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Abstract
The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, and with a novel clinical syndrome of impaired cognition and memory disturbance among humans after presumptive toxin exposure. As a result, there is a pressing need to better characterize the organism and these associations. Advances in Pfiesteria research have been hampered, however, by the absence of genomic sequence data. We employed a sequencing strategy directed by heteroduplex mobility assay to detect Pfiesteria piscicida 18S rDNA "signature" sequences in complex pools of DNA and used those data as the basis for determination of the complete P. piscicida 18S rDNA sequence. Specific PCR assays for P. piscicida and other estuarine heterotrophic dinoflagellates were developed, permitting their detection in algal cultures and in estuarine water samples collected during fish kill and fish lesion events. These tools should enhance efforts to characterize these organisms and their ecological relationships. Heteroduplex mobility assay-directed sequence discovery is broadly applicable, and may be adapted for the detection of genomic sequence data of other novel or nonculturable organisms in complex assemblages.
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