ArticleProceedings of the National Academy of Sciences of the United States of America2000
Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 204 citations in OpenAlex.
- Opposing effects on regulated insulin secretion of acute vs chronic stimulation of AMP-activated protein kinase.Diabetologia · 2022Article
- The Glucagon-Like Adipokinetic Hormone inFrontiers in physiology · 2021Review
- AMPK Profiling in Rodent and Human Pancreatic Beta-Cells under Nutrient-Rich Metabolic Stress.International journal of molecular sciences · 2020Article
- Article
- Insulin signaling in LepR cells modulates fat and glucose homeostasis independent of leptin.American journal of physiology. Endocrinology and metabolism · 2019Article
- Nutritional Modulation of AMPK-Impact upon Metabolic-Inflammation.International journal of molecular sciences · 2018Review
- AICAR suppresses TNF-α-induced complement factor B in RPE cells.Scientific reports · 2017Article
- Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation.International journal of molecular medicine · 2017Article
- Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.Molecular human reproduction · 2017Article
- Article
- Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.Diabetologia · 2016Article
- Reduced AMPKα2 protein expression restores glucose-induced insulin secretion in islets from calorie-restricted rats.International journal of experimental pathology · 2016Article
- LKB1 and AMPKα1 are required in pancreatic alpha cells for the normal regulation of glucagon secretion and responses to hypoglycemia.Molecular metabolism · 2015Article
- Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.Scientific reports · 2014Article
- Connexin36 contributes to INS-1E cells survival through modulation of cytokine-induced oxidative stress, ER stress and AMPK activity.Cell death and differentiation · 2013Article
- Article
- AMPK is involved in the regulation of incretin receptors expression in pancreatic islets under a low glucose concentration.PloS one · 2013Article
- Effect of 24 h Fasting on Gene Expression of AMPK, Appetite Regulation Peptides and Lipometabolism Related Factors in the Hypothalamus of Broiler Chicks.Asian-Australasian journal of animal sciences · 2012Article
- AMP-activated protein kinase, stress responses and cardiovascular diseases.Clinical science (London, England : 1979) · 2012Review
- Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast.Cell research · 2012Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Elevated glucose concentrations stimulate the transcription of the pre-proinsulin (PPI), L-type pyruvate kinase (L-PK), and other genes in islet beta cells. In liver cells, pharmacological activation by 5-amino-4-imidazolecarboxamide riboside (AICAR) of AMP-activated protein kinase (AMPK), the mammalian homologue of the yeast SNF1 kinase complex, inhibits the effects of glucose, suggesting a key signaling role for this kinase. Here, we demonstrate that AMPK activity is inhibited by elevated glucose concentrations in MIN6 beta cells and that activation of the enzyme with AICAR prevents the activation of the L-PK gene by elevated glucose. Furthermore, microinjection of antibodies to the alpha2- (catalytic) or beta2-subunits of AMPK complex, but not to the alpha1-subunit or extracellular stimulus-regulated kinase, mimics the effects of elevated glucose on the L-PK and PPI promoter activities as assessed by single-cell imaging of promoter luciferase constructs. In each case, injection of antibodies into the nucleus and cytosol, but not the nucleus alone, was necessary, indicating the importance of either a cytosolic phosphorylation event or the subcellular localization of the alpha2-subunits. Incubation with AICAR diminished, but did not abolish, the effect of glucose on PPI transcription. These data suggest that glucose-induced changes in AMPK activity are necessary and sufficient for the regulation of the L-PK gene by the sugar and also play an important role in the regulation of the PPI promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.