ArticleThe Plant cell2000
yellow-in-the-dark mutants of Chlamydomonas lack the CHLL subunit of light-independent protochlorophyllide reductase.
Article in The Plant cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 90 citations in OpenAlex.
- Turn the light on! Using algae to learn how chloroplasts are built.Plant physiology · 2026Article
- The yellow-in-the-dark chIL Chlamydomonas mutant as a model for time-resolved chloroplast biogenesis and retrograde signaling.Plant physiology · 2026Article
- MsmiR171 targetsHorticulture research · 2026Article
- Comparative analysis of chloroplast genomes of Loropetalum chinense and its purplish-red-flowered variety.Functional & integrative genomics · 2025Article
- Hidden diversity: Transcriptomic and photosynthetic variation among common 'wild type' Chlamydomonas strains.The Plant journal : for cell and molecular biology · 2025Article
- Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.Plant physiology · 2025Article
- Mutagenesis selection and large-scale cultivation of non-greenFrontiers in nutrition · 2024Article
- Article
- Plastid Genome Evolution of Two Colony-Forming BenthicInternational journal of molecular sciences · 2023Article
- Comparison ofLife (Basel, Switzerland) · 2022Article
- SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit.Horticulture research · 2021Article
- Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae.International journal of molecular sciences · 2021Review
- Metabolic engineering of ketocarotenoids biosynthetic pathway in Chlamydomonas reinhardtii strain CC-4102.Scientific reports · 2020Article
- Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.The Plant cell · 2019Article
- A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Evolution of light-independent protochlorophyllide oxidoreductase.Protoplasma · 2019Review
- The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants.Plant physiology · 2019Article
- SlARF10, an auxin response factor, is involved in chlorophyll and sugar accumulation during tomato fruit development.Journal of experimental botany · 2018Article
- New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii.Applied microbiology and biotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Light-independent protochlorophyllide reduction leading to chlorophyll formation in the dark requires both chloroplast and nuclear gene expression in Chlamydomonas reinhardtii. Mutations in any one of the plastid (chlL, chlN, and chlB) or nuclear (y-1 to y-10) genes required for this process result in the phenotype of the yellow-in-the-dark or y mutants. Analysis of the chlL, chlN, and chlB transcript levels in both light- and dark-grown wild-type and y mutant cells showed that the y mutations have no effect on the transcription of these plastid genes. Protein gel blot analysis showed that the CHLN and CHLB proteins are present in similar amounts in light- and dark-grown wild-type cells, whereas CHLL is present only in wild-type cells grown in the dark or at light intensities < or =15 micromol m(-2) sec(-1). Analysis of chlL transcript distribution on polysome profiles and rates of protein turnover in chloramphenicol-treated cells suggested that CHLL formation is most probably blocked at translation initiation or elongation. Furthermore, treatment of cells with metabolic inhibitors and uncouplers of photosynthetic electron transport showed that regulation of CHLL formation is linked to the physiologic status of the chloroplast. Similar to wild-type cells, y mutants contain nearly identical amounts of CHLN and CHLB when grown in either light or darkness. However, no CHLL is present in any of the y mutants except y-7, which contains an immunoreactive CHLL smaller than the expected size. Our findings indicate that CHLL translation is negatively photoregulated by the energy state or redox potential within the chloroplast in wild-type cells and that nuclear y genes are required for synthesis or accumulation of the CHLL protein.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.