ArticlePlant physiology2000
Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 206 citations in OpenAlex.
- Identification and validation of functional markers for the superoxide dismutase gene TaSOD-B2 in common wheat.BMC plant biology · 2025Article
- Combined transcriptomic and proteomic analysis reveals the response mechanisms of alfalfa to freezing stress.Frontiers in plant science · 2025Article
- Superoxide Dismutase Premodulates Oxidative Stress in Plastids for Protection of Tobacco Plants from Cold Damage Ultrastructure Damage.International journal of molecular sciences · 2024Article
- Research on drought stress inFrontiers in plant science · 2024Review
- Mnsod1 promotes the development of Pleurotus ostreatus and enhances the tolerance of mycelia to heat stress.Microbial cell factories · 2022Article
- Salicylic Acid Stimulates Defense Systems inMolecules (Basel, Switzerland) · 2022Article
- Pyramiding ascorbate-glutathione pathway in Lycopersicum esculentum confers tolerance to drought and salinity stress.Plant cell reports · 2022Article
- Recent advancement in OMICS approaches to enhance abiotic stress tolerance in legumes.Frontiers in plant science · 2022Review
- Article
- Cyclophilin OsCYP20-2 with a novel variant integrates defense and cell elongation for chilling response in rice.The New phytologist · 2020Article
- Deciphering hydrogen peroxide-induced signalling towards stress tolerance in plants.3 Biotech · 2019Review
- A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function.Horticulture research · 2018Article
- Improving the Yield and Nutritional Quality of Forage Crops.Frontiers in plant science · 2018Review
- A review of redox signaling and the control of MAP kinase pathway in plants.Redox biology · 2017Review
- Overproduction of superoxide dismutase and catalase confers cassava resistance to Tetranychus cinnabarinus.Scientific reports · 2017Article
- Tolerance analysis of chloroplast OsCu/Zn-SOD overexpressing rice under NaCl and NaHCO3 stress.PloS one · 2017Article
- Brassica RNA binding protein ERD4 is involved in conferring salt, drought tolerance and enhancing plant growth in Arabidopsis.Plant molecular biology · 2016Article
- Enhancing Heat Tolerance of the Little Dogwood Cornus canadensis L. f. with Introduction of a Superoxide Reductase Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus.Frontiers in plant science · 2016Article
- Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.Plant biotechnology journal · 2015Article
- Mitogen-activated protein kinase kinase 5 (MKK5)-mediated signalling cascade regulates expression of iron superoxide dismutase gene in Arabidopsis under salinity stress.Journal of experimental botany · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine whether overexpression of Fe-superoxide (SOD) dismutase would increase superoxide-scavenging capacity and thereby improve the winter survival of transgenic alfalfa (Medicago sativa L.) plants, two genotypes were transformed with the vector pEXSOD10, which contains a cDNA for Arabidopsis Fe-SOD with a chloroplast transit peptide and cauliflower mosaic virus 35S promoter. A novel Fe-SOD was detected by native PAGE in both greenhouse- and field-grown transgenic plants, but activity varied among independent transgenic plants. The increased Fe-SOD activity was associated with increased winter survival over 2 years in field trials, but not with oxidative stress tolerance as measured by resistance of leaves to methyl viologen, a superoxide generator. Total shoot dry matter production over 2 harvest years was not associated with Fe-SOD activity. There was no detectable difference in the pattern of primary freezing injury, as shown by vital staining, nor was there additional accumulation of carbohydrates in field-acclimated roots of the transgenic alfalfa plants. We did not detect any difference in growth of one transgenic plant with high Fe-SOD activity compared with a non-transgenic control. Therefore, the improvement in winter survival did not appear to be a consequence of improved oxidative stress tolerance associated with photosynthesis, nor was it a consequence of a change in primary freezing injury. We suggest that Fe-SOD overexpression reduced secondary injury symptoms and thereby enhanced recovery from stresses experienced during winter.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.