ArticlePlant physiology2000
Purified gamma-glutamyl transpeptidases from tomato exhibit high affinity for glutathione and glutathione S-conjugates.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 81 citations in OpenAlex.
- Glutathione Contribution in Interactions betweenInternational journal of molecular sciences · 2023Article
- Glutathione Modulation in PVYInternational journal of molecular sciences · 2022Article
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- The regulation of ethylene biosynthesis: a complex multilevel control circuitry.The New phytologist · 2021Review
- Effects of GGT and C-S Lyase on the Generation of Endogenous Formaldehyde inMolecules (Basel, Switzerland) · 2019Article
- Identification of Azoxystrobin Glutathione Conjugate Metabolites in Maize Roots by LC-MS.Molecules (Basel, Switzerland) · 2019Article
- Characterization of γ-glutamyltranspeptidases from dormant garlic and onion bulbs.Food science & nutrition · 2019Article
- 1-Aminocyclopropane 1-Carboxylic Acid and Its Emerging Role as an Ethylene-Independent Growth Regulator.Frontiers in plant science · 2019Review
- Functional Characterization of the Grapevine γ-Glutamyl Transferase/Transpeptidase (E.C. 2.3.2.2) Gene Family Reveals a Single Functional Gene Whose Encoded Protein Product Is Not Located in Either the Vacuole or Apoplast.Frontiers in plant science · 2019Article
- Article
- Comparative transcriptome analysis of dikaryotic mycelia and mature fruiting bodies in the edible mushroom Lentinula edodes.Scientific reports · 2018Article
- Effective production of S-allyl-L-cysteine through a homogeneous reaction with activated endogenous γ-glutamyltranspeptidase in garlic (Allium Sativum).Journal of food science and technology · 2015Article
- Compartment-specific importance of glutathione during abiotic and biotic stress.Frontiers in plant science · 2014Article
- 1-aminocyclopropane-1-carboxylic acid (ACC) in plants: more than just the precursor of ethylene!Frontiers in plant science · 2014Review
- Garlic γ-glutamyl transpeptidases that catalyze deglutamylation of biosynthetic intermediate of alliin.Frontiers in plant science · 2014Article
- Effect of pH on the hydrolytic kinetics of gamma-glutamyl transferase from Bacillus subtilis.TheScientificWorldJournal · 2014Article
- γ-Glutamyltranspeptidases: sequence, structure, biochemical properties, and biotechnological applications.Cellular and molecular life sciences : CMLS · 2012Review
- Experimental evidence for the involvement of amino acid residue Glu398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase.FEBS open bio · 2012Article
- Subcellular distribution of glutathione precursors in Arabidopsis thaliana.Journal of integrative plant biology · 2011Article
- Glutathione.The arabidopsis book · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
gamma-Glutamyl transpeptidases (gammaGTases) are the only enzymes known to hydrolyze the unique N-terminal amide bonds of reduced glutathione (gamma-L-glutamyl-cysteinyl-glycine), oxidized glutathione, and glutathione S-conjugates. Two gammaGTases (I and II) with K(m) values for glutathione of 110 and 90 microM were purified 2,977-fold and 2,152-fold, respectively, from ripe tomato (Lycopersicon esculentum) pericarp. Both enzymes also hydrolyze dipeptides and other tripeptides with N-terminal, gamma-linked Glu and the artificial substrates gamma-L-glutamyl-p-nitroanilide and gamma-L-glutamyl(7-amido-4-methylcoumarin). They transfer the glutamyl moiety to water or acceptor amino acids, including L-Met, L-Phe, L-Trp, L-Ala, or the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. gammaGTase I and II were released from a wall and membrane fraction of a tomato fruit extract with 1.0 M NaCl, suggesting that they are peripheral membrane proteins. They were further purified by acetone precipitation, Dye Matrex Green A affinity chromatography, and hydrophobic interaction chromatography. The two gammaGTases were resolved by concanavalin A (Con A) affinity chromatography, indicating that they are differentially glycosylated. The native and SDS-denatured forms of both enzymes showed molecular masses of 43 kD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.