ArticlePlant physiology2000
ATMPK4, an Arabidopsis homolog of mitogen-activated protein kinase, is activated in vitro by AtMEK1 through threonine phosphorylation.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 143 citations in OpenAlex.
- The Arabidopsis PM19L1 Protein Functions as a Regulator of Germination Under Osmotic Stress.Plant direct · 2025Article
- Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity.Plant biotechnology journal · 2023Article
- TheInternational journal of molecular sciences · 2022Article
- S-Nitrosation ofFrontiers in plant science · 2022Article
- Article
- Review
- PoplarInternational journal of molecular sciences · 2020Article
- Post-Translational Modifications of Proteins Have Versatile Roles in Regulating Plant Immune Responses.International journal of molecular sciences · 2019Review
- Mitogen-activated protein kinases MPK4 and MPK12 are key components mediating COThe Plant journal : for cell and molecular biology · 2018Article
- MAP kinase signalling: interplays between plant PAMP- and effector-triggered immunity.Cellular and molecular life sciences : CMLS · 2018Review
- Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling.Frontiers in plant science · 2018Review
- New functions of an old kinase MPK4 in guard cells.Plant signaling & behavior · 2018Article
- Analysis of MAP kinase MPK4/MEKK1/MKK genes of Carica papaya L. comparative to other plant homologues.Bioinformation · 2017Article
- Functional Involvement of a Mitogen Activated Protein Kinase Module, OsMKK3-OsMPK7-OsWRK30 in Mediating Resistance against Xanthomonas oryzae in Rice.Scientific reports · 2016Article
- Cooperative interaction of antimicrobial peptides with the interrelated immune pathways in plants.Molecular plant pathology · 2016Review
- Rhizobiales-like Phosphatase 2 from Arabidopsis thaliana Is a Novel Phospho-tyrosine-specific Phospho-protein Phosphatase (PPP) Family Protein Phosphatase.The Journal of biological chemistry · 2016Article
- Abiotic Stresses Antagonize the Rice Defence Pathway through the Tyrosine-Dephosphorylation of OsMPK6.PLoS pathogens · 2015Article
- Members of the Plant CRK Superfamily Are Capable of Trans- and Autophosphorylation of Tyrosine Residues.The Journal of biological chemistry · 2015Article
- AtDsPTP1 acts as a negative regulator in osmotic stress signalling during Arabidopsis seed germination and seedling establishment.Journal of experimental botany · 2015Article
- The pearl millet mitogen-activated protein kinase PgMPK4 is involved in responses to downy mildew infection and in jasmonic- and salicylic acid-mediated defense.Plant molecular biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modulation of mitogen-activated protein kinase (MAPK) activity regulates many intracellular signaling processes. In animal and yeast cells, MAP kinases are activated via phosphorylation by the dual-specificity kinase MEK (MAP kinase kinase). Several plant homologs of MEK and MAPK have been identified, but the biochemical events underlying the activation of plant MAPKs remain unknown. We describe the in vitro activation of an Arabidopsis homolog of MAP kinase, ATMPK4. ATMPK4 was phosphorylated in vitro by an Arabidopsis MEK homolog, AtMEK1. This phosphorylation occurred principally on threonine (Thr) residues and resulted in elevated ATMPK4 kinase activity. A second Arabidopsis MEK isoform, ATMAP2Kalpha, failed to phosphorylate ATMPK4 in vitro. Tyr dephosphorylation by the Arabidopsis Tyr-specific phosphatase AtPTP1 resulted in an almost complete loss of ATMPK4 activity. Immunoprecipitates of Arabidopsis extracts with anti-ATMPK4 antibodies displayed myelin basic protein kinase activity that was sensitive to treatment with AtPTP1. These results demonstrate that a plant MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants. Surprisingly, in contrast to the animal enzymes, AtMEK1 may not be a dual-specificity kinase but, rather, the required Tyr phosphorylation on ATMPK4 may result from autophosphorylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.