ArticlePlant physiology2000
Removal of feedback inhibition of delta(1)-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
174 citing papers in PubMed, 799 citations in OpenAlex.
- Evolutionary and Structural Insights into Proline Metabolism Genes Associated with Salt Resilience in Mango.Biochemical genetics · 2026Article
- Nano-Molybdenum Disulfide Enhances Antioxidant Defense and Aroma Formation in Fragrant Rice Under Cadmium Stress via Modulation of 2-Acetyl-1-Pyrroline Biosynthesis.Antioxidants (Basel, Switzerland) · 2026Article
- Amino Acids as Multifunctional Molecules in Plants: From Fundamental Metabolism to Precision Agriculture.Plants (Basel, Switzerland) · 2026Review
- Oxidative stress and osmolyte induction caused by a Thiobencarb nano-emulsion in the freshwater alga Chlorella vulgaris.Ecotoxicology (London, England) · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Impact of cultivar on grapevine physiological adaptation to drought and subsequent rehydration.Frontiers in plant science · 2026Article
- Genome-wide characterization of theFrontiers in plant science · 2026Article
- Morphological and biochemical responses to water stress inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2026Article
- Genome-Wide Characterization, Expression, and Functional Analysis of Acyl-CoA-Binding Protein (ACBP) Gene Family inPlants (Basel, Switzerland) · 2025Article
- Proline Tagging for Stress Tolerance in Plants.International journal of genomics · 2025Review
- The Class I HD-ZIP transcription factor PagHB7a functions as a positive regulator of salt tolerance inForestry research · 2025Article
- Proline and ROS: A Unified Mechanism in Plant Development and Stress Response?Plants (Basel, Switzerland) · 2024Review
- Characterization of a Drought-Induced Betaine Aldehyde Dehydrogenase GenePlants (Basel, Switzerland) · 2024Article
- Cytokinin oxidase2-deficient mutants improve panicle and grain architecture through cytokinin accumulation and enhance drought tolerance in indica rice.Plant cell reports · 2024Article
- Can Environmental Stressors Determine the Condition of Ecological Plant Groups?Plants (Basel, Switzerland) · 2024Article
- Introgression of ΔTransgenic research · 2024Article
- Chitosan from Mushroom Improves Drought Stress Tolerance in Tomatoes.Plants (Basel, Switzerland) · 2024Article
- Maize ZmLAZ1-3 gene negatively regulates drought tolerance in transgenic Arabidopsis.BMC plant biology · 2024Article
- UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts.The Plant cell · 2024Article
- Chilling stress response in tobacco seedlings: insights from transcriptome, proteome, and phosphoproteome analyses.Frontiers in plant science · 2024Article
114 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Delta(1)-pyrroline-5-carboxylate synthetase (P5CS; EC not assigned) is the rate-limiting enzyme in proline (Pro) biosynthesis in plants and is subject to feedback inhibition by Pro. It has been suggested that the feedback regulation of P5CS is lost in plants under stress conditions. We compared Pro levels in transgenic tobacco (Nicotiana tabacum) plants expressing a wild-type form of Vigna aconitifolia P5CS and a mutated form of the enzyme (P5CSF129A) whose feedback inhibition by Pro was removed by site-directed mutagenesis. Transgenic plants expressing P5CSF129A accumulated about 2-fold more Pro than the plants expressing V. aconitifolia wild-type P5CS. This difference was further increased in plants treated with 200 mM NaCl. These results demonstrated that the feedback regulation of P5CS plays a role in controlling the level of Pro in plants under both normal and stress conditions. The elevated Pro also reduced free radical levels in response to osmotic stress, as measured by malondialdehyde production, and significantly improved the ability of the transgenic seedlings to grow in medium containing up to 200 mM NaCl. These findings shed new light on the regulation of Pro biosynthesis in plants and the role of Pro in reducing oxidative stress induced by osmotic stress, in addition to its accepted role as an osmolyte.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.