ArticlePlant physiology2000
The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 1 of them a synthesis that pooled it.
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Who cites it
97 citing papers in PubMed, 1 synthesis or guideline pooled it, 303 citations in OpenAlex.
- Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives.Frontiers in plant science · 2022Pooled it
- Desiccation tolerance mechanisms in the green lineage and beyond.The New phytologist · 2026Review
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- Exploring the mechanisms of desert plant adaptation to arid climates: a multi-omics analysis of dehydration and rehydration responses in Syntrichia caninervis.Stress biology · 2025Article
- Heterologous WatermelonCurrent issues in molecular biology · 2025Article
- Genome-wide association study reveals the QTLs and candidate genes associated with seed longevity in soybean (Glycine max (L.) Merrill).BMC plant biology · 2025Article
- Towards resilience: Transcriptional insights on flavonoid biosynthesis during peanut seed maturation phases.PloS one · 2025Article
- Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem-preferred transgene expression.Frontiers in plant science · 2025Article
- Reveal the kernel dehydration mechanisms in maize based on proteomic and metabolomic analysis.BMC plant biology · 2024Article
- Tropical peanut maturation scale for harvesting seeds with superior quality.Frontiers in plant science · 2024Article
- WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition.The Biochemical journal · 2023Article
- Transcriptome analysis of thermomorphogenesis in ovules and during early seed development in Brassica napus.BMC genomics · 2023Article
- Intra-Specific Variation in Desiccation Tolerance ofInternational journal of molecular sciences · 2023Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Characterization ofFrontiers in genetics · 2023Article
- Priming Treatments with Biostimulants to Cope the Short-Term Heat Stress Response: A Transcriptomic Profile Evaluation.Plants (Basel, Switzerland) · 2022Article
- Overexpression ofCells · 2022Article
- Molecular Aspects of Seed Development Controlled by Gibberellins and Abscisic Acids.International journal of molecular sciences · 2022Review
- Updated role of ABA in seed maturation, dormancy, and germination.Journal of advanced research · 2022Review
- Proteomic Analysis of Embryo Isolated From MatureFrontiers in plant science · 2022Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To learn more about the function and regulation of small heat shock proteins (sHSPs) during seed development, we studied sHSP expression in wild-type and seed maturation mutants of Arabidopsis by western analysis and using an HSP17.4 promoter-driven beta-glucuronidase (GUS) reporter gene in transgenic plants. In the absence of stress, GUS activity increases during development until the entire embryo is stained before desiccation. Heat-stressed embryos stained for GUS at all stages, including early stages that showed no detectable HSP17. 4::GUS activity without heat. Examination of HSP17.4 expression in seeds of the transcriptional activator mutants abi3-6, fus3-3 (AIMS no. CS8014/N8014), and lec1-2 (AIMS no. CS2922/N2922) showed that protein and HSP17.4::GUS activity were highly reduced in fus3-3 and lec1-2 and undetectable in abi3-6 seeds. In contrast, heat-stressed abi3-6, fus3-3, and lec1-2 seeds stained for GUS activity throughout the embryo. These data indicate that there is distinct developmental and stress regulation of HSP17.4, and imply that ABI3 activates HSP17.4 transcription during development. Quantitation of sHSP protein in desiccation-intolerant seeds of abi3-6, fus3-3, lec1-2, and line24 showed that all had <2% of wild-type HSP17.4 levels. In contrast, the desiccation-tolerant but embryo-defective mutants emb266 (AIMS no. CS3049/N3049) and lec2-1 (AIMS no. CS2728/N2728) had wild-type levels of HSP17.4. These data correlate a reduction in sHSPs with desiccation intolerance and suggest that sHSPs have a general protective role throughout the seed.
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