ArticleMolecular and cellular biology2000
Identification and characterization of a novel serine-arginine-rich splicing regulatory protein.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- SR proteins in cancer: function, regulation, and small inhibitor.Cellular & molecular biology letters · 2024Review
- Characterization of M116.1p, a Murine Cytomegalovirus Protein Required for Efficient Infection of Mononuclear Phagocytes.Journal of virology · 2022Article
- Pathogenic SREK1 decrease in Huntington's disease lowers TAF1 mimicking X-linked dystonia parkinsonism.Brain : a journal of neurology · 2020Article
- Acute Simian Varicella Virus Infection Causes Robust and Sustained Changes in Gene Expression in the Sensory Ganglia.Journal of virology · 2016Article
- The RNA Splicing Response to DNA Damage.Biomolecules · 2015Review
- Splicosomal and serine and arginine-rich splicing factors as targets for TGF-β.Fibrogenesis & tissue repair · 2012Article
- Molecular characterization and expression patterns of serine/arginine-rich specific kinase 3 (SPRK3) in porcine skeletal muscle.Molecular biology reports · 2011Article
- Growth hormone deficiency and splicing fidelity: two serine/arginine-rich proteins, ASF/SF2 and SC35, act antagonistically.The Journal of biological chemistry · 2008Article
- NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells.Journal of virology · 2007Article
- A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation.The EMBO journal · 2007Article
- SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.Molecular and cellular biology · 2006Article
- XE7: a novel splicing factor that interacts with ASF/SF2 and ZNF265.Nucleic acids research · 2006Article
- Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.Molecular biology of the cell · 2006Article
- A novel SR-related protein is required for the second step of Pre-mRNA splicing.Molecular and cellular biology · 2005Article
- Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo.Molecular and cellular biology · 2004Article
- Regulation of alternative splicing by SRrp86 and its interacting proteins.Molecular and cellular biology · 2003Article
- B-cell and plasma-cell splicing differences: a potential role in regulated immunoglobulin RNA processing.RNA (New York, N.Y.) · 2003Article
- PSF and p54nrb bind a conserved stem in U5 snRNA.RNA (New York, N.Y.) · 2002Article
- A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1.Molecular biology of the cell · 2002Article
- Regulation of alternative splicing by SRrp86 through coactivation and repression of specific SR proteins.RNA (New York, N.Y.) · 2002Article
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Authors and funding
2 authors.
Funding
Abstract
We have identified an 86-kDa protein containing a single amino-terminal RNA recognition motif and two carboxy-terminal domains rich in serine-arginine (SR) dipeptides. Despite structural similarity to members of the SR protein family, p86 is clearly unique. It is not found in standard SR protein preparations, does not precipitate in the presence of high magnesium concentrations, is not recognized by antibodies specific for SR proteins, and cannot complement splicing-defective S100 extracts. However, we have found that p86 can inhibit the ability of purified SR proteins to activate splicing in S100 extracts and can even inhibit the in vitro and in vivo activation of specific splice sites by a subset of SR proteins, including ASF/SF2, SC35, and SRp55. In contrast, p86 activates splicing in the presence of SRp20. Thus, it appears that pairwise combination of p86 with specific SR proteins leads to altered splicing efficiency and differential splice site selection. In all cases, such regulation requires the presence of the two RS domains and a unique intervening EK-rich region, which appear to mediate direct protein-protein contact between these family members. Full-length p86, but not a mutant lacking the RS-EK-RS domains, was found to preferentially interact with itself, SRp20, ASF/SF2, SRp55, and, to a slightly lesser extent, SC35. Because of the primary sequence and unique properties of p86, we have named this protein SRrp86 for SR-related protein of 86 kDa.
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