ArticleMolecular and cellular biology2000
A novel cold-sensitive allele of the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme A carboxylase, affects the morphology of the yeast vacuole through acylation of Vac8p.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Shortening of membrane lipid acyl chains compensates for phosphatidylcholine deficiency in choline-auxotroph yeast.The EMBO journal · 2021Article
- ARMC Subfamily: Structures, Functions, Evolutions, Interactions, and Diseases.Frontiers in molecular biosciences · 2021Review
- Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms ofFrontiers in microbiology · 2020Article
- Article
- The role of phospholipid molecular species in determining the physical properties of yeast membranes.FEBS letters · 2018Review
- Analysis of Arabidopsis Accessions Hypersensitive to a Loss of Chloroplast Translation.Plant physiology · 2016Article
- Genetic analysis of the Warburg effect in yeast.Advances in biological regulation · 2015Review
- Transcriptome response to alkane biofuels in Saccharomyces cerevisiae: identification of efflux pumps involved in alkane tolerance.Biotechnology for biofuels · 2013Article
- Exposure to fine airborne particulate matter induces macrophage infiltration, unfolded protein response, and lipid deposition in white adipose tissue.American journal of translational research · 2013Article
- Review
- Peroxisomes are involved in biotin biosynthesis in Aspergillus and Arabidopsis.The Journal of biological chemistry · 2011Article
- Requirement for acetyl-CoA carboxylase in Trypanosoma brucei is dependent upon the growth environment.Molecular microbiology · 2011Article
- Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae.Biotechnology for biofuels · 2010Article
- Rab-regulated interaction of early endosomes with lipid droplets.Biochimica et biophysica acta · 2007Article
- The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.The EMBO journal · 2004Article
- Inhibition of acetyl coenzyme A carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Vac8p release from the SNARE complex and its palmitoylation are coupled and essential for vacuole fusion.The EMBO journal · 2001Article
- Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The yeast vacuole functions both as a degradative organelle and as a storage depot for small molecules and ions. Vacuoles are dynamic reticular structures that appear to alternately fuse and fragment as a function of growth stage and environment. Vac8p, an armadillo repeat-containing protein, has previously been shown to function both in vacuolar inheritance and in protein targeting from the cytoplasm to the vacuole. Both myristoylation and palmitoylation of Vac8p are required for its efficient localization to the vacuolar membrane (Y.-X. Wang, N. L. Catlett, and L. S. Weisman, J. Cell Biol. 140:1063-1074, 1998). We report that mutants with conditional defects in the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme A carboxylase (ACC1), display unusually multilobed vacuoles, similar to those observed in vac8 mutant cells. This vacuolar phenotype of acc1 mutant cells was shown biochemically to be accompanied by a reduced acylation of Vac8p which was alleviated by fatty acid supplementation. Consistent with the proposed defect of acc1 mutant cells in acylation of Vac8p, vacuolar membrane localization of Vac8p was impaired upon shifting acc1 mutant cells to nonpermissive condition. The function of Vac8p in protein targeting, on the other hand, was not affected under these conditions. These observations link fatty acid synthesis and availability to direct morphological alterations of an organellar membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.