ArticleGenetics2000
Subtelomeric repeat amplification is associated with growth at elevated temperature in yku70 mutants of Saccharomyces cerevisiae.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii.FEMS yeast research · 2024Article
- Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.G3 (Bethesda, Md.) · 2021Article
- Article
- A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii.Synthetic biology (Oxford, England) · 2021Article
- The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.Genetics · 2014Article
- Mec1p associates with functionally compromised telomeres.Chromosoma · 2012Article
- A naturally thermolabile activity compromises genetic analysis of telomere function in Saccharomyces cerevisiae.Genetics · 2012Article
- SUMOylation regulates telomere length homeostasis by targeting Cdc13.Nature structural & molecular biology · 2011Article
- Est1 protects telomeres and inhibits subtelomeric y'-element recombination.Molecular and cellular biology · 2011Article
- Article
- Taming the tiger by the tail: modulation of DNA damage responses by telomeres.The EMBO journal · 2009Review
- Sensitivity of yeast strains with long G-tails to levels of telomere-bound telomerase.PLoS genetics · 2007Article
- Repair of cyclobutane pyrimidine dimers or dimethylsulfate damage in DNA is identical in normal or telomerase-immortalized human skin fibroblasts.Nucleic acids research · 2005Article
- Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86.Molecular and cellular biology · 2004Article
- Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends.Genes & development · 2003Article
- Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer.Nucleic acids research · 2003Article
- The Rad51 pathway of telomerase-independent maintenance of telomeres can amplify TG1-3 sequences in yku and cdc13 mutants of Saccharomyces cerevisiae.Molecular and cellular biology · 2003Article
- EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants.Genes & development · 2002Article
- Telomere length deregulation and enhanced sensitivity to genotoxic stress in Arabidopsis mutants deficient in Ku70.The EMBO journal · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inactivation of the Saccharomyces cerevisiae gene YKU70 (HDF1), which encodes one subunit of the Ku heterodimer, confers a DNA double-strand break repair defect, shortening of and structural alterations in the telomeres, and a severe growth defect at 37 degrees. To elucidate the basis of the temperature sensitivity, we analyzed subclones derived from rare yku70 mutant cells that formed a colony when plated at elevated temperature. In all these temperature-resistant subclones, but not in cell populations shifted to 37 degrees, we observed substantial amplification and redistribution of subtelomeric Y' element DNA. Amplification of Y' elements and adjacent telomeric sequences has been described as an alternative pathway for chromosome end stabilization that is used by postsenescence survivors of mutants deficient for the telomerase pathway. Our data suggest that the combination of Ku deficiency and elevated temperature induces a potentially lethal alteration of telomere structure or function. Both in yku70 mutants and in wild type, incubation at 37 degrees results in a slight reduction of the mean length of terminal restriction fragments, but not in a significant loss of telomeric (C(1-3)A/TG(1-3))(n) sequences. We propose that the absence of Ku, which is known to bind to telomeres, affects the telomeric chromatin so that its chromosome end-defining function is lost at 37 degrees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.