ArticleNucleic acids research2000
Fission yeast hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- mRNA stability in the nucleus.Journal of Zhejiang University. Science. B · 2014Review
- CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe.The EMBO journal · 2012Article
- Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin.The EMBO journal · 2012Article
- SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae.PLoS genetics · 2012Article
- Distinct chromatin modulators regulate the formation of accessible and repressive chromatin at the fission yeast recombination hotspot ade6-M26.Molecular biology of the cell · 2008Article
- Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity.Molecular biology of the cell · 2007Article
- The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.Nucleic acids research · 2005Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- Identification and analysis of chromodomain-containing proteins encoded in the mouse transcriptome.Genome research · 2003Article
- Rye terminal neocentromeres: characterisation of the underlying DNA and chromatin structure.Chromosoma · 2003Article
- Gain- and loss-of-function of Rhp51, a Rad51 homolog in fission yeast, reveals dissimilarities in chromosome integrity.Nucleic acids research · 2001Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hrp1 of Schizosaccharomyces pombe is a member of the CHD protein family, characterized by a chromodomain, a Myb-like telobox-related DNA-binding domain and a SNF2-related helicase/ATPase domain. CHD proteins are thought to be required for modification of the chromatin structure in transcription, but the exact roles of CHD proteins are not known. Here we examine the sub-cellular localization and biochemical activity of Hrp1 and the phenotypes of hrp1 Delta and Hrp1-overexpressing strains. Fluorescence microscopy revealed that Hrp1 protein is targeted to the nucleus. We found that Hrp1 exhibited DNA-dependent ATPase activity, stimulated by both single- and double-stranded DNA. Overexpression of Hrp1 caused slow cell growth accompanied by defective chromosome condensation in anaphase resulting in a 'cut' (celluntimelytorn) phenotype and chromosome loss. The hrp1 Delta mutation also caused abnormal anaphase and mini-chromosome loss phenotypes. Electron micrographs demonstrated that aberrantly shaped nucleoli appeared in Hrp1-overexpressing cells. Therefore, these results suggest that Hrp1 may play a role in mitotic chromosome segregation and maintenance of chromatin structure by utilizing the energy from ATP hydrolysis.
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