ArticleNucleic acids research2000
MeCP2 driven transcriptional repression in vitro: selectivity for methylated DNA, action at a distance and contacts with the basal transcription machinery.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- MeCP2 functions as a DNA methylation reader in immune homeostasis and cancer.Frontiers in epigenetics and epigenomics · 2026Review
- CRL4Cellular and molecular life sciences : CMLS · 2024Article
- Article
- MiR-422a promotes adipogenesis via MeCP2 downregulation in human bone marrow mesenchymal stem cells.Cellular and molecular life sciences : CMLS · 2023Article
- The Chromatin Structure at theInternational journal of molecular sciences · 2022Article
- DNA Methylation in Nonalcoholic Fatty Liver Disease.International journal of molecular sciences · 2020Review
- The role of MeCP2 in learning and memory.Learning & memory (Cold Spring Harbor, N.Y.) · 2019Article
- Interleukin-Mediated Pendrin Transcriptional Regulation in Airway and Esophageal Epithelia.International journal of molecular sciences · 2019Review
- Genomic and regulatory characteristics of significant transcription factors in colorectal cancer metastasis.Scientific reports · 2018Article
- The effects of biological aging on global DNA methylation, histone modification, and epigenetic modifiers in the mouse germinal vesicle stage oocyte.Animal reproduction · 2018Article
- S-Adenosyl Methionine and Transmethylation Pathways in Neuropsychiatric Diseases Throughout Life.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2018Review
- Epigenetics and Liver Fibrosis.Cellular and molecular gastroenterology and hepatology · 2017Review
- Anticancer Natural Compounds as Epigenetic Modulators of Gene Expression.Current genomics · 2017Review
- MECP2 missense mutations outside the canonical MBD and TRD domains in males with intellectual disability.Journal of human genetics · 2016Article
- Modifiers and Readers of DNA Modifications and Their Impact on Genome Structure, Expression, and Stability in Disease.Frontiers in genetics · 2016Review
- Chromatin context and ncRNA highlight targets of MeCP2 in brain.RNA biology · 2013Article
- Biochemical analysis of histone deacetylase-independent transcriptional repression by MeCP2.The Journal of biological chemistry · 2013Article
- A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.PloS one · 2013Article
- Association of modified cytosines and the methylated DNA-binding protein MeCP2 with distinctive structural domains of lampbrush chromatin.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
- Coil-to-helix transitions in intrinsically disordered methyl CpG binding protein 2 and its isolated domains.Protein science : a publication of the Protein Society · 2012Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The pathways for selective transcriptional repression of methylated DNA templates by the methyl-CpG-binding protein MeCP2 have been investigated using a purified in vitro transcription system that does not assemble chromatin. MeCP2 selectively inhibits transcription complex assembly on methylated DNA but does not destabilize a pre-assembled transcription complex. MeCP2 functions to repress transcription at a distance of >500 bp from the transcription start site. The transcription repression domain (TRD) of MeCP2 will repress transcription in vitro when fused to a heterologous Gal4 DNA-binding domain. The TRD associates with TFIIB. Exogenous TFIIB does not relieve transcriptional repression established by either intact MeCP2 or a Gal4-TRD fusion protein under these in vitro conditions, nor does the addition of histone deacetylase inhibitors. We find that the transcriptional repression established by both MeCP2 and the Gal4-TRD fusion protein in vitro also correlates with selective assembly of large nucleoprotein complexes. The formation of such complexes reflects a local concentration of DNA-bound transcriptional repressor that may stabilize a state of repression even in the presence of exogenous transcriptional machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.