ArticleMolecular vision2000
Increased expression of osteonectin/SPARC mRNA and protein in age-related human cataracts and spatial expression in the normal human lens.
Article in Molecular vision, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed, 49 citations in OpenAlex.
- Exosomal microRNA-222-3p increases UVB sensitivity of lens epithelium cells by suppressing MGMT.International ophthalmology · 2023Article
- Thrombospondin-1 Signaling Through the Calreticulin/LDL Receptor Related Protein 1 Axis: Functions and Possible Roles in Glaucoma.Frontiers in cell and developmental biology · 2022Review
- Mutations of RagA GTPase in mTORC1 Pathway Are Associated with Autosomal Dominant Cataracts.PLoS genetics · 2016Article
- Secreted Protein Acidic and Rich in Cysteine in Ocular Tissue.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2015Review
- Matricellular proteins in the trabecular meshwork: review and update.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2014Review
- Can proteomics yield insight into aging aorta?Proteomics. Clinical applications · 2013Review
- Secreted protein acidic and rich in cysteine (SPARC)-null mice exhibit more uniform outflow.Investigative ophthalmology & visual science · 2013Article
- Thrombospondin-1 (TSP1)-null and TSP2-null mice exhibit lower intraocular pressures.Investigative ophthalmology & visual science · 2012Article
- Pax6 interacts with SPARC and TGF-β in murine eyes.Molecular vision · 2012Article
- Mutational screening of VSX1, SPARC, SOD1, LOX, and TIMP3 in keratoconus.Molecular vision · 2011Article
- Evaluation of SPARC as a candidate gene of juvenile-onset primary open-angle glaucoma by mutation and copy number analyses.Molecular vision · 2010Article
- Genome-wide transcription analysis of histidine-related cataract in Atlantic salmon (Salmo salar L).Molecular vision · 2009Article
- Identification and functional clustering of global gene expression differences between age-related cataract and clear human lenses and aged human lenses.Experimental eye research · 2004Review
- Methionine sulfoxide reductase A is important for lens cell viability and resistance to oxidative stress.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
purposeWe have previously reported increased levels of Osteonectin/SPARC transcript in age-related cataractous compared to normal human lenses. The purpose of the present study was to evaluate the corresponding levels of osteonectin/SPARC protein in age-related cataractous relative to normal lenses and to evaluate the levels of osteonectin/SPARC transcript in specific types of age-related human cataracts. The spatial expression of osteonectin/SPARC was also evaluated in normal human lenses.
methodsSpecific types of age-related cataracts were collected and graded. Normal human lenses were microdissected into epithelia and fibers. Osteonectin/SPARC protein levels were monitored by Western immunoblotting, and transcript levels were evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Osteonectin/SPARC expression patterns were examined by RT-PCR and by immunostaining.
resultsHigher levels of osteonectin/SPARC protein were detected in age-related cataractous relative to normal human lenses. Increased levels of osteonectin/SPARC transcript were also detected in posterior-subcapsular and nuclear cataractous lenses relative to normal lenses. Osteonectin/SPARC transcripts were detected in the lens epithelium but not fibers. Osteonectin/SPARC protein levels were highest in the peripheral lens epithelium.
conclusionsConsistent with our previous studies on osteonectin/SPARC mRNA levels, osteonectin/SPARC protein levels were also elevated in cataractous compared to normal human lenses. Increased levels of osteonectin/SPARC mRNA were also found in nuclear and posterior-subcapsular cataracts relative to normal lenses. Osteonectin/SPARC expression is confined to the lens epithelium, and osteonectin/SPARC levels are highest in the peripheral lens epithelium.
Indexed as
Identifiers
10756178PMC2831409W309654497What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.