ArticleJournal of virology2000
Cultured cell sublines highly susceptible to prion infection.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 212 citations in OpenAlex.
- A novel ER stress regulator ARL6IP5 induces reticulophagy to ameliorate the prion burden.Autophagy · 2025Article
- Genetically engineered cellular models of prion propagation.Cell and tissue research · 2023Review
- Review
- Propagation and Dissemination Strategies of Transmissible Spongiform Encephalopathy Agents in Mammalian Cells.International journal of molecular sciences · 2022Review
- An astrocyte cell line that differentially propagates murine prions.The Journal of biological chemistry · 2020Article
- Prion infection, transmission, and cytopathology modeled in a low-biohazard human cell line.Life science alliance · 2020Article
- Global analysis of protein degradation in prion infected cells.Scientific reports · 2020Article
- Article
- Review
- A Bioluminescent Cell Assay to Quantify Prion Protein Dimerization.Scientific reports · 2018Article
- Prion-like properties of disease-relevant proteins in amyotrophic lateral sclerosis.Journal of neural transmission (Vienna, Austria : 1996) · 2018Review
- Bioassays and Inactivation of Prions.Cold Spring Harbor perspectives in biology · 2017Review
- PrP Knockout Cells Expressing Transmembrane PrP Resist Prion Infection.Journal of virology · 2017Article
- Transcriptomic Determinants of Scrapie Prion Propagation in Cultured Ovine Microglia.PloS one · 2016Article
- Synthetic prions with novel strain-specified properties.PLoS pathogens · 2015Article
- Review
- Article
- Evaluation of infective property of recombinant prion protein amyloids in cultured cells overexpressing cellular prion protein.Journal of Korean medical science · 2014Article
- Assessing proteinase K resistance of fish prion proteins in a scrapie-infected mouse neuroblastoma cell line.Viruses · 2014Article
- Identification of a gene regulatory network associated with prion replication.The EMBO journal · 2014Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured cell lines infected with prions produce an abnormal isoform of the prion protein (PrP(Sc)). In order to derive cell lines producing sufficient quantities of PrP(Sc) for most studies, it has been necessary to subclone infected cultures and select the subclones producing the largest amounts of PrP(Sc). Since postinfection cloning can introduce differences between infected and uninfected cell lines, we sought an approach to generate prion-infected cell lines that would avoid clonal artifacts. Using an improved cell blot technique, which permits sensitive and rapid comparison of PrP(Sc) levels in multiple independent cell cultures, we discovered marked heterogeneity with regard to prion susceptibility in tumor cell sublines. We exploited this heterogeneity to derive sublines which are highly susceptible to prion infection and used these cells to generate prion-infected lines without further subcloning. These infected sublines can be compared to the cognate uninfected cultures without interference from cloning artifacts. We also used susceptible cell lines and our modified cell blot procedure to develop a sensitive and reproducible quantitative cell culture bioassay for prions. We found that the sublines were at least 100-fold more susceptible to strain RML prions than to strain ME7 prions. Comparisons between scrapie-susceptible and -resistant cell lines may reveal factors that modulate prion propagation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.