ArticleJournal of virology2000
Subgenomic negative-strand RNA function during mouse hepatitis virus infection.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed.
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- The journey of SARS-CoV-2 in human hosts: a review of immune responses, immunosuppression, and their consequences.Virulence · 2021Review
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- In search of RdRp and Mpro inhibitors against SARS CoV-2: Molecular docking, molecular dynamic simulations and ADMET analysis.Journal of molecular structure · 2021Article
- Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA.Journal of virological methods · 2021Article
- The human pandemic coronaviruses on the show: The spike glycoprotein as the main actor in the coronaviruses play.International journal of biological macromolecules · 2021Review
- A nidovirus perspective on SARS-CoV-2.Biochemical and biophysical research communications · 2021Review
- Translational control of coronaviruses.Nucleic acids research · 2020Review
- Insights into the Recent 2019 Novel Coronavirus (SARS-CoV-2) in Light of Past Human Coronavirus Outbreaks.Pathogens (Basel, Switzerland) · 2020Review
- A 21st Century Evil: Immunopathology and New Therapies of COVID-19.Frontiers in immunology · 2020Review
- Managing the COVID-19 Pandemic: Research Strategies Based on the Evolutionary and Molecular Characteristics of Coronaviruses.SN comprehensive clinical medicine · 2020Review
- Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus.Virology · 2019Article
- The structure and functions of coronavirus genomic 3' and 5' ends.Virus research · 2015Review
- Coronavirus pathogenesis.Advances in virus research · 2011Review
- Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures.Journal of virology · 2010Article
- Subgenomic messenger RNA amplification in coronaviruses.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Recombination, reservoirs, and the modular spike: mechanisms of coronavirus cross-species transmission.Journal of virology · 2010Review
- Arterivirus Nsp1 modulates the accumulation of minus-strand templates to control the relative abundance of viral mRNAs.PLoS pathogens · 2010Article
- SARS-coronavirus replication/transcription complexes are membrane-protected and need a host factor for activity in vitro.PLoS pathogens · 2008Article
- Structural lability in stem-loop 1 drives a 5' UTR-3' UTR interaction in coronavirus replication.Journal of molecular biology · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Mouse hepatitis virus (MHV)-infected cells contain full-length and subgenomic-length positive- and negative-strand RNAs. The origin and function of the subgenomic negative-strand RNAs is controversial. In this report we demonstrate that the synthesis and molar ratios of subgenomic negative strands are similar in alternative host cells, suggesting that these RNAs function as important mediators of positive-strand synthesis. Using kinetic labeling experiments, we show that the full-length and subgenomic-length replicative form RNAs rapidly accumulate and then saturate with label, suggesting that the subgenomic-length negative strands are the principal mediators of positive-strand synthesis. Using cycloheximide, which preferentially inhibits negative-strand and to a lesser extent positive-strand synthesis, we demonstrate that cycloheximide treatment equally inhibits full-length and subgenomic-length negative-strand synthesis. Importantly, following treatment, previously transcribed negative strands remain in transcriptionally active complexes even in the absence of new negative-strand synthesis. These findings indicate that the subgenomic-length negative strands are the principal templates of positive-strand synthesis during MHV infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.